Protein Info for b1386 in Escherichia coli BW25113
Name: tynA
Annotation: tyramine oxidase, copper-requiring (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to AMO_ECOLI: Primary amine oxidase (tynA) from Escherichia coli (strain K12)
KEGG orthology group: K00276, primary-amine oxidase [EC: 1.4.3.21] (inferred from 100% identity to eco:b1386)MetaCyc: 100% identical to copper-containing amine oxidase (Escherichia coli K-12 substr. MG1655)
Primary-amine oxidase. [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]; 1.4.3.21 [EC: 1.4.3.21, 1.4.3.4]
Predicted SEED Role
"Monoamine oxidase (1.4.3.4)" in subsystem Aromatic Amin Catabolism or Auxin biosynthesis or Glycine and Serine Utilization or Threonine degradation
MetaCyc Pathways
- superpathway of phenylethylamine degradation (11/11 steps found)
- superpathway of L-threonine metabolism (16/18 steps found)
- L-threonine degradation III (to methylglyoxal) (3/3 steps found)
- phenylethylamine degradation I (2/2 steps found)
- putrescine degradation III (2/4 steps found)
- salidroside biosynthesis (2/4 steps found)
- phenylethanol biosynthesis (2/5 steps found)
- L-tryptophan degradation X (mammalian, via tryptamine) (1/4 steps found)
- serotonin degradation (3/7 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (4/9 steps found)
- dopamine degradation (1/5 steps found)
- aromatic biogenic amine degradation (bacteria) (2/8 steps found)
- noradrenaline and adrenaline degradation (4/13 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis I
- Alkaloid biosynthesis II
- Arginine and proline metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Drug metabolism - cytochrome P450
- Glycine, serine and threonine metabolism
- Histidine metabolism
- Phenylalanine metabolism
- Tryptophan metabolism
- Tyrosine metabolism
- Urea cycle and metabolism of amino groups
- beta-Alanine metabolism
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.4.3.21 or 1.4.3.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P46883 at UniProt or InterPro
Protein Sequence (757 amino acids)
>b1386 tyramine oxidase, copper-requiring (NCBI) (Escherichia coli BW25113) MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTLKEFGADVQWDDYAQLF TLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSDTFINDVFQSGLDQTFQVE KRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLPPDKEAVWAFALENKPVDQPR KADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVLLDDFASVQNIINNSEEFAAAVK KRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVISYLDVGDGNYWAHPIENLVAVVDL EQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVKPMQIIEPEGKNYTITGDMIHWRNWDF HLSMNSRVGPMISTVTYNDNGTKRKVMYEGSLGGMIVPYGDPDIGWYFKAYLDSGDYGMG TLTSPIARGKDAPSNAVLLNETIADYTGVPMEIPRAIAVFERYAGPEYKHQEMGQPNVST ERRELVVRWISTVGNYDYIFDWIFHENGTIGIDAGATGIEAVKGVKAKTMHDETAKDDTR YGTLIDHNIVGTTHQHIYNFRLDLDVDGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNI GNEQDAAQKFDPGTIRLLSNPNKENRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHR LSFMDKQLWVTRYHPGERFPEGKYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHV ARAEEWPIMPTEWVHTLLKPWNFFDETPTLGALKKDK