Protein Info for b1378 in Escherichia coli BW25113

Name: ydbK
Annotation: fused predicted pyruvate-flavodoxin oxidoreductase: conserved protein/conserved protein/FeS binding protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02176: pyruvate:ferredoxin (flavodoxin) oxidoreductase
  • amino acids 1 to 1165 (1165 residues), 1626.1 bits, E=0
• PF01855: POR_N
  • amino acids 13 to 243 (231 residues), 275.7 bits, E=2.3e-85
• PF17147: PFOR_II
  • amino acids 265 to 347 (83 residues), 58.2 bits, 6e-19
• PF01558: POR
  • amino acids 426 to 609 (184 residues), 122.9 bits, E=1.1e-38
• PF10371: EKR
  • amino acids 626 to 678 (53 residues), 84.5 bits, see alignment (E = 2.4e-27)
• PF00037: Fer4
  • amino acids 683 to 705 (23 residues), 23.9 bits, see alignment (E = 1.8e-08)
  • amino acids 740 to 757 (18 residues), 22.1 bits, see alignment (E = 6.8e-08)
• PF13237: Fer4_10
  • amino acids 685 to 756 (72 residues), 30.2 bits, 2.3e-10
• PF13183: Fer4_8
  • amino acids 688 to 758 (71 residues), 27.3 bits, 2.9e-09
• PF12838: Fer4_7
  • amino acids 689 to 757 (69 residues), 39.5 bits, 4.2e-13
• PF13534: Fer4_17
  • amino acids 689 to 757 (69 residues), 23.4 bits, 4.5e-08
• PF13484: Fer4_16
  • amino acids 689 to 757 (69 residues), 35.7 bits, 8.4e-12
• PF13187: Fer4_9
  • amino acids 689 to 758 (70 residues), 30.8 bits, 1.5e-10

Best Hits

Swiss-Prot: 100% identical to NIFJ_ECOLI: Probable pyruvate-flavodoxin oxidoreductase (ydbK) from Escherichia coli (strain K12)

KEGG orthology group: K03737, putative pyruvate-flavodoxin oxidoreductase [EC: 1.2.7.-] (inferred from 75% identity to aha:AHA_3174)

MetaCyc: 100% identical to putative pyruvate-flavodoxin oxidoreductase (Escherichia coli K-12 substr. MG1655)
Pyruvate synthase. [EC: 1.2.7.1]

Predicted SEED Role

"Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-)" in subsystem Methionine Degradation or Pyruvate:ferredoxin oxidoreductase (EC 1.2.7.-)

MetaCyc Pathways

• superpathway of fermentation (Chlamydomonas reinhardtii) (8/9 steps found)
• pyruvate fermentation to acetate and lactate II (4/4 steps found)
• pyruvate fermentation to acetate I (3/3 steps found)
• pyruvate fermentation to acetate VII (3/3 steps found)
• pyruvate fermentation to ethanol III (3/3 steps found)
• L-alanine degradation V (oxidative Stickland reaction) (2/2 steps found)
• reductive monocarboxylic acid cycle (2/2 steps found)
• pyruvate decarboxylation to acetyl CoA III (1/1 steps found)
• pyruvate fermentation to hexanol (engineered) (8/11 steps found)
• reductive TCA cycle I (8/11 steps found)
• incomplete reductive TCA cycle (5/7 steps found)
• pyruvate fermentation to acetate VI (2/3 steps found)
• pyruvate fermentation to acetate and alanine (2/3 steps found)
• purine nucleobases degradation II (anaerobic) (17/24 steps found)
• pyruvate fermentation to acetate III (1/2 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• glycerol degradation to butanol (11/16 steps found)
• pyruvate fermentation to acetone (3/5 steps found)
• reductive TCA cycle II (8/12 steps found)
• lactate fermentation to acetate, CO₂ and hydrogen (Desulfovibrionales) (5/8 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• superpathway of L-alanine fermentation (Stickland reaction) (5/9 steps found)
• isopropanol biosynthesis (engineered) (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (7/13 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (9/17 steps found)
• L-glutamate degradation VII (to butanoate) (5/12 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (22/56 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Propanoate metabolism
• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Trinitrotoluene degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.7.- or 1.2.7.1

Sequence Analysis Tools

See P52647 at UniProt or InterPro

Protein Sequence (1174 amino acids)

>b1378 fused predicted pyruvate-flavodoxin oxidoreductase: conserved protein/conserved protein/FeS binding protein (NCBI) (Escherichia coli BW25113)
MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGLKNVWGDTPRVVEMQSE
AGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTPFVLHVAARTVATHALSIF
GDHSDVMAVRQTGCAMLCAANVQEAQDFALISQIATLKSRVPFIHFFDGFRTSHEINKIV
PLADDTILDLMPQVEIDAHRARALNPEHPVIRGTSANPDTYFQSREATNPWYNAVYDHVE
QAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAIGTCEEVVDELLTRGEKVGVLKVRL
YRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAEPLYLDVMTALAEAFNNGERETLPRVI
GGRYGLSSKEFGPDCVLAVFAELNAAKPKARFTVGIYDDVTNLSLPLPENTLPNSAKLEA
LFYGLGSDGSVSATKNNIKIIGNSTPWYAQGYFVYDSKKAGGLTVSHLRVSEQPIRSAYL
ISQADFVGCHQLQFIDKYQMAERLKPGGIFLLNTPYSADEVWSRLPQEVQAVLNQKKARF
YVINAAKIARECGLAARINTVMQMAFFHLTQILPGDSALAELQGAIAKSYSSKGQDLVER
NWQALALARESVEEVPLQPVNPHSANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALP
PDGTWPMGTTRWEKRNIAEEIPIWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPAS
LHSLDVKSRDMRGQKYVLQVAPEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEE
KINYDFFLNLPEIDRSKLERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRML
IANATGCSSIYGGNLPSTPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRL
LDQFADKIPAELLTALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLI
GGDGWAYDIGFGGLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRK
ARKDLGVSMMMYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLAL
SHDQMRQLTATGFWPLYRFDPRRADEGKLPLALDSRPPSEAPEETLLHEQRFRRLNSQQP
EVAEQLWKDAAADLQKRYDFLAQMAGKAEKSNTD