Protein Info for b1335 in Escherichia coli BW25113

Name: ogt
Annotation: O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 171 PF02870: Methyltransf_1N" amino acids 4 to 83 (80 residues), 71.5 bits, see alignment E=8.8e-24 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 87 to 166 (80 residues), 129 bits, see alignment E=2.6e-42 PF01035: DNA_binding_1" amino acids 88 to 167 (80 residues), 127 bits, see alignment E=2e-41

Best Hits

Swiss-Prot: 100% identical to OGT_ECOLI: Methylated-DNA--protein-cysteine methyltransferase (ogt) from Escherichia coli (strain K12)

KEGG orthology group: K00567, methylated-DNA-[protein]-cysteine S-methyltransferase [EC: 2.1.1.63] (inferred from 100% identity to eco:b1335)

MetaCyc: 100% identical to methylated-DNA--[protein]-cysteine S-methyltransferase (Escherichia coli K-12 substr. MG1655)
Methylated-DNA--[protein]-cysteine S-methyltransferase. [EC: 2.1.1.63]; 2.1.1.63 [EC: 2.1.1.63]

Predicted SEED Role

"Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63)" in subsystem DNA repair, bacterial (EC 2.1.1.63)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.63

Use Curated BLAST to search for 2.1.1.63

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AFH0 at UniProt or InterPro

Protein Sequence (171 amino acids)

>b1335 O-6-alkylguanine-DNA:cysteine-protein methyltransferase (NCBI) (Escherichia coli BW25113)
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
GLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGA
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL