Protein Info for b1288 in Escherichia coli BW25113
Name: fabI
Annotation: enoyl-(acyl carrier protein) reductase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to FABI_SHIFL: Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (fabI) from Shigella flexneri
KEGG orthology group: K00208, enoyl-[acyl-carrier protein] reductase I [EC: 1.3.1.9] (inferred from 100% identity to eco:b1288)MetaCyc: 100% identical to enoyl-[acyl-carrier-protein] reductase (Escherichia coli K-12 substr. MG1655)
Enoyl-[acyl-carrier-protein] reductase (NADH). [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; 1.3.1.9 [EC: 1.3.1.9]; Enoyl-[acyl-carrier-protein] reductase (NADPH, B-specific). [EC: 1.3.1.9, 1.3.1.10, 1.3.1.104, 1.3.1.39]
Predicted SEED Role
"Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.3.1.9)
MetaCyc Pathways
- superpathway of fatty acids biosynthesis (E. coli) (51/53 steps found)
- superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
- palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
- superpathway of unsaturated fatty acids biosynthesis (E. coli) (20/20 steps found)
- superpathway of fatty acid biosynthesis I (E. coli) (16/16 steps found)
- biotin biosynthesis I (15/15 steps found)
- 8-amino-7-oxononanoate biosynthesis I (11/11 steps found)
- oleate biosynthesis IV (anaerobic) (13/14 steps found)
- palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
- (5Z)-dodecenoate biosynthesis I (6/6 steps found)
- cis-vaccenate biosynthesis (5/5 steps found)
- fatty acid elongation -- saturated (5/5 steps found)
- gondoate biosynthesis (anaerobic) (4/4 steps found)
- (5Z)-dodecenoate biosynthesis II (5/6 steps found)
- stearate biosynthesis II (bacteria and plants) (5/6 steps found)
- 8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
- octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (9/12 steps found)
- palmitate biosynthesis III (21/29 steps found)
- stearate biosynthesis IV (4/6 steps found)
- anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
- even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
- odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
- tetradecanoate biosynthesis (mitochondria) (17/25 steps found)
- streptorubin B biosynthesis (20/34 steps found)
- mycolate biosynthesis (25/205 steps found)
- superpathway of mycolate biosynthesis (26/239 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.3.1.10 or 1.3.1.104 or 1.3.1.39 or 1.3.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P0AEK4 at UniProt or InterPro
Protein Sequence (262 amino acids)
>b1288 enoyl-(acyl carrier protein) reductase (NCBI) (Escherichia coli BW25113) MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIV LQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDIS SYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPE GVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGI SGEVVHVDGGFSIAAMNELELK