Protein Info for b1197 in Escherichia coli BW25113

Name: treA
Annotation: periplasmic trehalase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF01204: Trehalase" amino acids 55 to 532 (478 residues), 613.4 bits, see alignment E=3.8e-188 PF06202: GDE_C" amino acids 170 to 441 (272 residues), 34.1 bits, see alignment E=1.6e-12

Best Hits

Swiss-Prot: 100% identical to TREA_ECODH: Periplasmic trehalase (treA) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K01194, alpha,alpha-trehalase [EC: 3.2.1.28] (inferred from 100% identity to eco:b1197)

MetaCyc: 100% identical to periplasmic trehalase (Escherichia coli K-12 substr. MG1655)
Alpha,alpha-trehalase. [EC: 3.2.1.28]

Predicted SEED Role

No annotation

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.28

Use Curated BLAST to search for 3.2.1.28

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P13482 at UniProt or InterPro

Protein Sequence (565 amino acids)

>b1197 periplasmic trehalase (NCBI) (Escherichia coli BW25113)
MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILLGPLFNDVQNAKLFPDQ
KTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPKEGEKYVPPEGQSLREHID
GLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWDSYFTMLGLAESGHWDKVADM
VANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELLAQHEGDAALKQYLPQMQKEYAY
WMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTPRPESWVEDIATAKSNPNRPATEIY
RDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVPVDLNSLMFKMEKILARASKAAGDNAM
ANQYETLANARQKGIEKYLWNDQQGWYADYDLKSHKVRNQLTAAALFPLYVNAAAKDRAN
KMATATKTHLLQPGGLNTTSVKSGQQWDAPNGWAPLQWVATEGLQNYGQKEVAMDISWHF
LTNVQHTYDREKKLVEKYDVSTTGTGGGGGEYPLQDGFGWTNGVTLKMLDLICPKEQPCD
NVPATRPTVKSATTQPSTKEAQPTP