Protein Info for b1190 in Escherichia coli BW25113
Name: dadX
Annotation: alanine racemase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to ALR2_ECOLI: Alanine racemase, catabolic (dadX) from Escherichia coli (strain K12)
KEGG orthology group: K01775, alanine racemase [EC: 5.1.1.1] (inferred from 100% identity to eco:b1190)MetaCyc: 100% identical to alanine racemase 2 (Escherichia coli K-12 substr. MG1655)
Alanine racemase. [EC: 5.1.1.1, 5.1.1.10]
Predicted SEED Role
"Alanine racemase (EC 5.1.1.1)" in subsystem Alanine biosynthesis or Pyruvate Alanine Serine Interconversions (EC 5.1.1.1)
MetaCyc Pathways
- L-alanine degradation I (2/2 steps found)
- alanine racemization (1/1 steps found)
- anaerobic energy metabolism (invertebrates, cytosol) (5/7 steps found)
- superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
- vancomycin resistance II (1/3 steps found)
- L-arginine degradation XIV (oxidative Stickland reaction) (1/6 steps found)
- L-lysine degradation V (2/9 steps found)
- ansatrienin biosynthesis (1/9 steps found)
- cyclosporin A biosynthesis (2/15 steps found)
- superpathway of L-lysine degradation (14/43 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Cysteine metabolism
- D-Alanine metabolism
- D-Arginine and D-ornithine metabolism
- D-Glutamine and D-glutamate metabolism
- Glycine, serine and threonine metabolism
Isozymes
Compare fitness of predicted isozymes for: 5.1.1.1
Use Curated BLAST to search for 5.1.1.1 or 5.1.1.10
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P29012 at UniProt or InterPro
Protein Sequence (356 amino acids)
>b1190 alanine racemase (NCBI) (Escherichia coli BW25113) MTRPIQASLDLQALKQNLSIVRQAATHARVWSVVKANAYGHGIERIWSAIGATDGFALLN LEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHSNWQLKALQNARLKAPLDI YLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHFAEAEHPDGISGAMARIEQAA EGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPSGQWRDIANTGLRPVMTLSSEII GVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGYPRHAPTGTPVLVDGVRTMTVGTVS MDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAAAAGTVGYELMCALALRVPVVTV