Protein Info for b1189 in Escherichia coli BW25113

Name: dadA
Updated annotation (from data): D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1)
Rationale: Specific phenotype: utilization of D-Alanine
Original annotation: D-amino acid dehydrogenase small subunit (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF00070: Pyr_redox" amino acids 2 to 33 (32 residues), 23.5 bits, see alignment (E = 2.7e-08) PF01266: DAO" amino acids 2 to 397 (396 residues), 275.7 bits, see alignment E=3.2e-85 PF02558: ApbA" amino acids 3 to 33 (31 residues), 22.8 bits, see alignment (E = 2.5e-08) PF13450: NAD_binding_8" amino acids 5 to 43 (39 residues), 25.9 bits, see alignment 4e-09

Best Hits

Swiss-Prot: 100% identical to DADA_ECOBW: D-amino acid dehydrogenase (dadA) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K00285, D-amino-acid dehydrogenase [EC: 1.4.99.1] (inferred from 100% identity to eco:b1189)

MetaCyc: 100% identical to D-amino acid dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-11193 [EC: 1.4.5.1]; 1.4.5.- [EC: 1.4.5.1]

Predicted SEED Role

"D-amino acid dehydrogenase small subunit (EC 1.4.99.1)" in subsystem Pyruvate Alanine Serine Interconversions or Respiratory dehydrogenases 1 (EC 1.4.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.5.1 or 1.4.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0A6J5 at UniProt or InterPro

Protein Sequence (432 amino acids)

>b1189 D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (Escherichia coli BW25113)
MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANAGQISPGYAAPWAAPGV
PLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYMENKGRMVRLAEYSRDCLK
ALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDAGVPYQLLESSRLAEVEPALA
EVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKFRFNTPVDQLLCDGEQIYGVKCG
DEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYSLTIPIAQEDGAPVSTILDETYKIA
ITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMVVRDLYPRGGHVEQATFWTGLRPMTPD
GTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQLLSDLLSGRTPAIPYEDLSVARYSRGFT
PSRPGHLHGAHS