Protein Info for b1184 in Escherichia coli BW25113

Name: umuC
Annotation: DNA polymerase V subunit UmuC (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 PF00817: IMS" amino acids 5 to 150 (146 residues), 145.7 bits, see alignment E=2.1e-46 PF11798: IMS_HHH" amino acids 172 to 200 (29 residues), 28.3 bits, see alignment (E = 2.8e-10) PF11799: IMS_C" amino acids 245 to 360 (116 residues), 85.9 bits, see alignment E=5.1e-28 PF13438: DUF4113" amino acids 371 to 421 (51 residues), 76.2 bits, see alignment 3.2e-25

Best Hits

Swiss-Prot: 100% identical to UMUC_ECOLI: Protein UmuC (umuC) from Escherichia coli (strain K12)

KEGG orthology group: K03502, DNA polymerase V (inferred from 100% identity to eco:b1184)

MetaCyc: 100% identical to DNA polymerase V catalytic protein (Escherichia coli K-12 substr. MG1655)
DNA-directed DNA polymerase. [EC: 2.7.7.7]

Predicted SEED Role

"Error-prone, lesion bypass DNA polymerase V (UmuC)"

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P04152 at UniProt or InterPro

Protein Sequence (422 amino acids)

>b1184 DNA polymerase V subunit UmuC (NCBI) (Escherichia coli BW25113)
MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEAKALGVKMGDPWFKQKD
LFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDEAFCDLTGVRNCRDLTDFG
REIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQTGGVVDLSNLERQRKLMSALP
VDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHFNVVLERTVRELRGEPCLQLEEF
APTKQEIICSRSFGERITDYPSMRQAICSYAARAAEKLRSEHQYCRFISTFIKTSPFALN
EPYYGNSASVKLLTPTQDSRDIINAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNL
FDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLR
VK