Protein Info for b1135 in Escherichia coli BW25113

Name: ymfC
Annotation: orf, hypothetical protein (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 41 to 186 (146 residues), 62.5 bits, E=2.7e-21
• TIGR00093: pseudouridine synthase
  • amino acids 45 to 217 (173 residues), 204.4 bits, E=4.5e-65

Best Hits

Swiss-Prot: 100% identical to RLUE_ECOLI: Ribosomal large subunit pseudouridine synthase E (rluE) from Escherichia coli (strain K12)

KEGG orthology group: K06181, ribosomal large subunit pseudouridine synthase E [EC: 5.4.99.12] (inferred from 100% identity to eco:b1135)

MetaCyc: 100% identical to 23S rRNA pseudouridine2457 synthase (Escherichia coli K-12 substr. MG1655)
RXN-11834 [EC: 5.4.99.20]

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70)" (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (2/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12 or 5.4.99.20

Sequence Analysis Tools

See P75966 at UniProt or InterPro

Protein Sequence (217 amino acids)

>b1135 orf, hypothetical protein (VIMSS) (Escherichia coli BW25113)
MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILFNKPYDVLPQFTDEAGR
KTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPGKRTGKIYYVQVEGIPTQD
ALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRERKSIPTSWLKITLYEGRNRQV
RRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD