Protein Info for b1134 in Escherichia coli BW25113

Name: ymfB
Annotation: bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00293: NUDIX
  • amino acids 4 to 115 (112 residues), 72.9 bits, E=1.3e-24

Best Hits

Swiss-Prot: 100% identical to NUDJ_ECODH: Phosphatase NudJ (nudJ) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K12152, phosphatase NudJ [EC: 3.6.1.-] (inferred from 100% identity to eco:b1134)

MetaCyc: 100% identical to phosphatase NudJ (Escherichia coli K-12 substr. MG1655)
RXN0-7309 [EC: 3.6.1.76]; Guanosine-diphosphatase. [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]; 3.6.1.- [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]; 3.6.1.- [EC: 3.6.1.76, 3.6.1.42, 3.6.1.5]

Predicted SEED Role

"Nudix-like NDP and NTP phosphohydrolase YmfB" in subsystem Nudix proteins (nucleoside triphosphate hydrolases)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• prenylated FMNH₂ biosynthesis (3/3 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Purine metabolism
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-

Use Curated BLAST to search for 3.6.1.- or 3.6.1.42 or 3.6.1.5 or 3.6.1.76

Sequence Analysis Tools

See P0AEI6 at UniProt or InterPro

Protein Sequence (153 amino acids)

>b1134 bifunctional thiamin pyrimidine pyrophosphate hydrolase/ thiamin pyrophosphate hydrolase (NCBI) (Escherichia coli BW25113)
MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETLVEAAARELWEETGISA
QPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDIDCCRWVSAEEILQASNLRS
PLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI