Protein Info for b1133 in Escherichia coli BW25113

Name: ycfB
Annotation: orf, hypothetical protein (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 368 PF02540: NAD_synthase" amino acids 3 to 75 (73 residues), 31.1 bits, see alignment E=3.3e-11 PF02568: ThiI" amino acids 4 to 75 (72 residues), 24.6 bits, see alignment E=4.4e-09 PF03054: tRNA_Me_trans" amino acids 6 to 202 (197 residues), 281.8 bits, see alignment E=8e-88 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 6 to 360 (355 residues), 534.3 bits, see alignment E=5.7e-165 PF20259: tRNA_Me_trans_M" amino acids 208 to 275 (68 residues), 84.1 bits, see alignment E=1e-27 PF20258: tRNA_Me_trans_C" amino acids 286 to 360 (75 residues), 96.2 bits, see alignment E=2.9e-31

Best Hits

Swiss-Prot: 100% identical to MNMA_ECODH: tRNA-specific 2-thiouridylase MnmA (mnmA) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 100% identity to eco:b1133)

MetaCyc: 100% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.- or 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P25745 at UniProt or InterPro

Protein Sequence (368 amino acids)

>b1133 orf, hypothetical protein (VIMSS) (Escherichia coli BW25113)
MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQA
VCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLG
ADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVRK
IAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQPGKIITVDGDEIGEHQGLMYHTL
GQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQGHEHPRLMSVGLIAQQLHWVDREPF
TGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFDEPVAAVTPGQSAVFYNGEVCLGGGII
EQRLPLPV