Protein Info for b1109 in Escherichia coli BW25113

Name: ndh
Annotation: respiratory NADH dehydrogenase 2/cupric reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF07992: Pyr_redox_2
  • amino acids 6 to 338 (333 residues), 219.1 bits, E=2.2e-68
• PF00070: Pyr_redox
  • amino acids 172 to 255 (84 residues), 40.1 bits, E=1.1e-13

Best Hits

Swiss-Prot: 100% identical to DHNA_ECOLI: NADH dehydrogenase (ndh) from Escherichia coli (strain K12)

KEGG orthology group: K03885, NADH dehydrogenase [EC: 1.6.99.3] (inferred from 100% identity to eco:b1109)

MetaCyc: 100% identical to NADH:quinone oxidoreductase II (Escherichia coli K-12 substr. MG1655)
RXN0-5330 [EC: 1.6.5.9]; 1.6.5.9 [EC: 1.6.5.9]; 1.16.1.- [EC: 1.6.5.9]

Predicted SEED Role

"NADH dehydrogenase (EC 1.6.99.3)" in subsystem Carboxysome or Respiratory dehydrogenases 1 (EC 1.6.99.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to cytochrome bd oxidase electron transfer II (2/2 steps found)
• NADH to nitrate electron transfer (2/2 steps found)
• nitrate reduction VIIIb (dissimilatory) (2/2 steps found)
• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (6/8 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NAD(P)/NADPH interconversion (4/6 steps found)
• NADH to cytochrome aa₃ oxidase electron transfer (1/2 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• NADH to cytochrome bo oxidase electron transfer II (1/2 steps found)
• mitochondrial NADPH production (yeast) (3/5 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• aerobic respiration II (cytochrome c) (yeast) (2/4 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.6.5.9 or 1.6.99.3

Sequence Analysis Tools

See P00393 at UniProt or InterPro

Protein Sequence (434 amino acids)

>b1109 respiratory NADH dehydrogenase 2/cupric reductase (NCBI) (Escherichia coli BW25113)
MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHSHLWKPLLHEVATGSLD
EGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDEKGELLVPERKIAYDTLVM
ALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLKYSANLGANGKVNIAIVGGGA
TGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGERILPALPPRISAAAHNELTKLG
VRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGIKAPDFLKDIGGLETNRINQLVVEP
TLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAAHQMATCAMNNILAQMNGKPLKNYQYK
DHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRIARFVYISLYRMHQIALHGYFKTGLMMLV
GSINRVIRPRLKLH