Protein Info for b0903 in Escherichia coli BW25113
Name: pflB
Annotation: pyruvate formate lyase I (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to PFLB_ECOLI: Formate acetyltransferase 1 (pflB) from Escherichia coli (strain K12)
KEGG orthology group: K00656, formate C-acetyltransferase [EC: 2.3.1.54] (inferred from 100% identity to ecp:ECP_0914)MetaCyc: 100% identical to pyruvate formate-lyase (Escherichia coli K-12 substr. MG1655)
Predicted SEED Role
"Pyruvate formate-lyase (EC 2.3.1.54)" in subsystem Butanol Biosynthesis or Fermentations: Mixed acid (EC 2.3.1.54)
MetaCyc Pathways
- superpathway of N-acetylneuraminate degradation (22/22 steps found)
- hexitol fermentation to lactate, formate, ethanol and acetate (19/19 steps found)
- mixed acid fermentation (16/16 steps found)
- superpathway of L-threonine metabolism (16/18 steps found)
- L-threonine degradation I (6/6 steps found)
- superpathway of fermentation (Chlamydomonas reinhardtii) (8/9 steps found)
- pyruvate fermentation to acetate IV (3/3 steps found)
- pyruvate fermentation to ethanol I (3/3 steps found)
- (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
- reductive monocarboxylic acid cycle (2/2 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.3.1.54
Use Curated BLAST to search for 2.3.1.54
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P09373 at UniProt or InterPro
Protein Sequence (760 amino acids)
>b0903 pyruvate formate lyase I (NCBI) (Escherichia coli BW25113) MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFLAGATEATTTLWDKVME GVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQTEAPLKRALIPFGGIKMIE GSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRCRKSGVLTGLPDAYGRGRIIG DYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTIRLREEIAEQHRALGQMKEMAAK YGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMSFGRTSTFLDVYIERDLKAGKITEQ EAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIWATESIGGMGLDGRTLVTKNSFRFLNT LYTMGPSPEPNMTILWSEKLPLNFKKFAAKVSIDTSSLQYENDDLMRPDFNNDDYAIACC VSPMIVGKQMQFFGARANLAKTMLYAINGGVDEKLKMQVGPKSEPIKGDVLNYDEVMERM DHFMDWLAKQYITALNIIHYMHDKYSYEASLMALHDRDVIRTMACGIAGLSVAADSLSAI KYAKVKPIRDEDGLAIDFEIEGEYPQFGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIP TQSVLTITSNVVYGKKTGNTPDGRRAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYA KDGISYTFSIVPNALGKDDEVRKTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAM ENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQSM