Protein Info for b0873 in Escherichia coli BW25113

Name: ybjW
Annotation: putative prismane (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 TIGR01703: hydroxylamine reductase" amino acids 1 to 548 (548 residues), 766.3 bits, see alignment E=7.3e-235 PF03063: Prismane" amino acids 1 to 545 (545 residues), 521.9 bits, see alignment E=9.5e-161

Best Hits

Swiss-Prot: 100% identical to HCP_ECOBW: Hydroxylamine reductase (hcp) from Escherichia coli (strain K12 / MC4100 / BW2952)

KEGG orthology group: K00378, hydroxylamine reductase [EC: 1.7.-.-] (inferred from 100% identity to eco:b0873)

MetaCyc: 100% identical to protein S-nitrosylase (Escherichia coli K-12 substr. MG1655)
Hydroxylamine reductase. [EC: 1.7.99.1]

Predicted SEED Role

"Hydroxylamine reductase (EC 1.7.-.-)" in subsystem Nitrosative stress (EC 1.7.-.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.-.-

Use Curated BLAST to search for 1.7.-.- or 1.7.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P75825 at UniProt or InterPro

Protein Sequence (550 amino acids)

>b0873 putative prismane (VIMSS) (Escherichia coli BW25113)
MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQGLSAWAVKAREYGIINH
DVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQCLAVDANARVDNPMADLQL
VSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLKGAAAYMEHAHVLGQYDNDIY
AQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSILDAGETGKYGHPTPTQVNVKATA
GKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLPAHGYPELRKFKHLVGNYGSGWQNQ
QVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRSIVGWPGVRHLDGDDFSAVITQAQQMA
GFPYSEIPHLITVGFGRQTLLGAADTLIDLVSREKLRHIFLLGGCDGARGERHYFTDFAT
SVPDDCLILTLACGKYRFNKLEFGDIEGLPRLVDAGQCNDAYSAIILAVTLAEKLGCGVN
DLPLSLVLSWFEQKAIVILLTLLSLGVKNIVTGPTAPGFLTPDLLAVLNEKFGLRSITTV
EEDMKQLLSA