Protein Info for b0822 in Escherichia coli BW25113

Name: ybiV
Annotation: predicted hydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 271 PF00702: Hydrolase" amino acids 3 to 226 (224 residues), 32.6 bits, see alignment E=1.6e-11 TIGR00099: Cof-like hydrolase" amino acids 5 to 259 (255 residues), 221.1 bits, see alignment E=1.8e-69 PF08282: Hydrolase_3" amino acids 6 to 259 (254 residues), 183.5 bits, see alignment E=9.4e-58 TIGR01484: HAD hydrolase, family IIB" amino acids 6 to 232 (227 residues), 76.1 bits, see alignment E=4.3e-25 PF05116: S6PP" amino acids 124 to 248 (125 residues), 46.1 bits, see alignment E=7.5e-16

Best Hits

Swiss-Prot: 100% identical to SUPH_ECOLI: Sugar phosphatase YbiV (ybiV) from Escherichia coli (strain K12)

KEGG orthology group: K07757, sugar-phosphatase [EC: 3.1.3.23] (inferred from 100% identity to eco:b0822)

MetaCyc: 100% identical to sugar phosphatase YbiV (Escherichia coli K-12 substr. MG1655)
Sugar-phosphatase. [EC: 3.1.3.23]

Predicted SEED Role

"HMP-PP hydrolase (pyridoxal phosphatase) Cof, detected in genetic screen for thiamin metabolic genes (PMID:15292217)"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.3.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P75792 at UniProt or InterPro

Protein Sequence (271 amino acids)

>b0822 predicted hydrolase (NCBI) (Escherichia coli BW25113)
MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVASGNQYYQLISFFPELKD
EISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQLNFVACGLQSAYVSENAPE
AFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPLVIDKLHVALDGIMKPVTSGF
GFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGNDAEMLKMARYSFAMGNAAENIK
QIARYATDDNNHEGALNVIQAVLDNTSPFNS