Protein Info for b0739 in Escherichia coli BW25113

Name: tolA
Annotation: cell envelope integrity inner membrane protein TolA (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 421 transmembrane" amino acids 13 to 33 (21 residues), see Phobius details TIGR02794: protein TolA" amino acids 12 to 245 (234 residues), 161.7 bits, see alignment E=1.5e-51 amino acids 232 to 421 (190 residues), 153.2 bits, see alignment E=5.6e-49 PF06519: TolA" amino acids 325 to 419 (95 residues), 130.9 bits, see alignment E=8e-43

Best Hits

Swiss-Prot: 100% identical to TOLA_ECOLI: Tol-Pal system protein TolA (tolA) from Escherichia coli (strain K12)

KEGG orthology group: K03646, colicin import membrane protein (inferred from 100% identity to eco:b0739)

Predicted SEED Role

"TolA protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P19934 at UniProt or InterPro

Protein Sequence (421 amino acids)

>b0739 cell envelope integrity inner membrane protein TolA (NCBI) (Escherichia coli BW25113)
MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGGGGGSSIDAVMVDSGAV
VEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQERLKQLEKERLAAQEQKKQA
EEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAAKKAAADAKKKAEAEAAKAAA
EAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKKAAAEKAAADKKAAA
EKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGE
LSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAG
KTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
P