Protein Info for b0732 in Escherichia coli BW25113

Name: mngB
Annotation: alpha-mannosidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 877 PF01074: Glyco_hydro_38N" amino acids 7 to 260 (254 residues), 135.5 bits, see alignment E=4.7e-43 PF09261: Alpha-mann_mid" amino acids 280 to 375 (96 residues), 45.3 bits, see alignment E=1.6e-15 PF07748: Glyco_hydro_38C" amino acids 490 to 706 (217 residues), 51 bits, see alignment E=4.7e-17 PF17677: Glyco_hydro38C2" amino acids 803 to 850 (48 residues), 23.8 bits, see alignment 7.4e-09

Best Hits

Swiss-Prot: 100% identical to MNGB_ECOLI: Mannosylglycerate hydrolase (mngB) from Escherichia coli (strain K12)

KEGG orthology group: K01191, alpha-mannosidase [EC: 3.2.1.24] (inferred from 100% identity to eco:b0732)

MetaCyc: 100% identical to alpha-mannosidase (Escherichia coli K-12 substr. MG1655)
3.2.1.-

Predicted SEED Role

"Alpha-mannosidase (EC 3.2.1.24)" in subsystem Fructooligosaccharides(FOS) and Raffinose Utilization or Mannose Metabolism (EC 3.2.1.24)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.2.1.24

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P54746 at UniProt or InterPro

Protein Sequence (877 amino acids)

>b0732 alpha-mannosidase (NCBI) (Escherichia coli BW25113)
MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQDNEYKYYVLDGQTAILE
DYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIVRNLMYGMRDCLAFGEPMK
IGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDKTEFLWQSSDGSEVTAQVLPL
GYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLPNGHDQMPLQQNIFEVMDKLREI
YPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGKYMRVHRTIGSTRMDIKIAHARIEN
KIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILKNHAHDSIGCCCSDKVHREIVARFELA
EDMADNLIRFYMRKIADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQ
PVPYFIRHAREIDPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVI
AAKSDAEGILENAFWQIALNEDGSLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEE
WVITAANAKPQCDIIHEAWQSRAVIRYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSR
RIDVDINLDNQADDHRLRVLVPTPFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAP
VPVWNMLNYVALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPG
RPSGIKMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDVMKL
NKAGFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVIS
CSETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA