Protein Info for b0661 in Escherichia coli BW25113

Name: miaB
Annotation: isopentenyl-adenosine A37 tRNA methylthiolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 PF00919: UPF0004" amino acids 4 to 104 (101 residues), 103.1 bits, see alignment E=1e-33 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 4 to 440 (437 residues), 738.5 bits, see alignment E=2.8e-226 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 437 (434 residues), 530.3 bits, see alignment E=3.4e-163 PF04055: Radical_SAM" amino acids 151 to 325 (175 residues), 97 bits, see alignment E=2.2e-31 PF01938: TRAM" amino acids 378 to 440 (63 residues), 73.8 bits, see alignment E=1.2e-24

Best Hits

Swiss-Prot: 100% identical to MIAB_ECODH: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Escherichia coli (strain K12 / DH10B)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 100% identity to eco:b0661)

MetaCyc: 100% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AEI1 at UniProt or InterPro

Protein Sequence (474 amino acids)

>b0661 isopentenyl-adenosine A37 tRNA methylthiolase (NCBI) (Escherichia coli BW25113)
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP