Protein Info for b0616 in Escherichia coli BW25113

Name: citE
Annotation: citrate lyase beta chain (acyl lyase subunit) (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 signal peptide" amino acids 16 to 32 (17 residues), see Phobius details amino acids 34 to 34 (1 residues), see Phobius details transmembrane" amino acids 33 to 33 (1 residues), see Phobius details TIGR01588: citrate (pro-3S)-lyase, beta subunit" amino acids 12 to 299 (288 residues), 451.9 bits, see alignment E=3.7e-140 PF03328: HpcH_HpaI" amino acids 15 to 234 (220 residues), 292.1 bits, see alignment E=2.2e-91 PF15617: C-C_Bond_Lyase" amino acids 210 to 299 (90 residues), 30.7 bits, see alignment E=1.9e-11

Best Hits

Swiss-Prot: 100% identical to CITE_ECOLI: Citrate lyase subunit beta (citE) from Escherichia coli (strain K12)

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 100% identity to eco:b0616)

MetaCyc: 100% identical to citrate lyase beta subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Citrate lyase beta chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 4.1.3.34 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0A9I1 at UniProt or InterPro

Protein Sequence (302 amino acids)

>b0616 citrate lyase beta chain (acyl lyase subunit) (VIMSS) (Escherichia coli BW25113)
MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLEDSVALREKDTARRMVY
HALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRLPKTDTAQDVLDIEKEILR
IEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGIALGAEDYVRNLRTERSPEGT
ELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEAAHIKQLGFDGKSLINPRQIDLL
HNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNGKMVDGPVIDRARLVLSRAELSGIR
EE