Protein Info for b0595 in Escherichia coli BW25113

Name: entB
Annotation: isochorismatase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00857: Isochorismatase
  • amino acids 32 to 204 (173 residues), 140.2 bits, E=7.8e-45
• PF00550: PP-binding
  • amino acids 217 to 276 (60 residues), 30.9 bits, E=2.7e-11

Best Hits

Swiss-Prot: 100% identical to ENTB_ECO57: Enterobactin synthase component B (entB) from Escherichia coli O157:H7

KEGG orthology group: K01252, enterobactin isochorismatase [EC: 3.3.2.1] (inferred from 100% identity to eco:b0595)

MetaCyc: 100% identical to enterobactin synthase component B (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• 2,3-dihydroxybenzoate biosynthesis (3/3 steps found)
• enterobactin biosynthesis (7/11 steps found)
• vibriobactin biosynthesis (4/9 steps found)
• bacillibactin biosynthesis (6/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Biosynthesis of siderophore group nonribosomal peptides

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.3.2.1

Sequence Analysis Tools

See P0ADI4 at UniProt or InterPro

Protein Sequence (285 amino acids)

>b0595 isochorismatase (NCBI) (Escherichia coli BW25113)
MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVSFWGENCPMMEQVIANI
AALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRSPEQQKVVDRLTPDADDTV
LVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTTATDAFMRDIKPFMVADALAD
FSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAALREVILPLLDESDEPFDDDNLID
YGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAWWKLLSREVK