Protein Info for b0594 in Escherichia coli BW25113

Name: entE
Annotation: 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02275: (2,3-dihydroxybenzoyl)adenylate synthase
  • amino acids 3 to 527 (525 residues), 939.8 bits, E=1.6e-287
• PF00501: AMP-binding
  • amino acids 37 to 395 (359 residues), 227.3 bits, E=2.8e-71
• PF13193: AMP-binding_C
  • amino acids 446 to 520 (75 residues), 46.5 bits, E=5.7e-16

Best Hits

Swiss-Prot: 100% identical to ENTE_ECOLI: Enterobactin synthase component E (entE) from Escherichia coli (strain K12)

KEGG orthology group: K02363, enterobactin 2,3-dihydroxybenzoate-AMP ligase / S-dihydroxybenzoyltransferase [EC: 2.3.1.- 2.7.7.58] (inferred from 100% identity to eco:b0594)

MetaCyc: 100% identical to 2,3-dihydroxybenzoate-AMP ligase (Escherichia coli K-12 substr. MG1655)
2,3-dihydroxybenzoate--serine ligase. [EC: 6.2.1.71, 6.3.2.14]; 2.7.7.- [EC: 6.2.1.71, 6.3.2.14]

Predicted SEED Role

"2,3-dihydroxybenzoate-AMP ligase (EC 2.7.7.58) [enterobactin] siderophore" in subsystem Siderophore Enterobactin (EC 2.7.7.58)

MetaCyc Pathways

• superpathway of chorismate metabolism (54/59 steps found)
• enterobactin biosynthesis (7/11 steps found)
• vibriobactin biosynthesis (4/9 steps found)
• bacillibactin biosynthesis (6/12 steps found)
• shinorine biosynthesis (3/8 steps found)
• vanchrobactin biosynthesis (3/8 steps found)
• acinetobactin biosynthesis (3/9 steps found)
• anguibactin biosynthesis (2/8 steps found)
• myxochelin A and B biosynthesis (2/9 steps found)
• colibactin biosynthesis (7/38 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Anthocyanin biosynthesis
• Benzoate degradation via CoA ligation
• Biosynthesis of siderophore group nonribosomal peptides
• Biosynthesis of terpenoids and steroids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of unsaturated fatty acids
• Butanoate metabolism
• Carotenoid biosynthesis - General
• Diterpenoid biosynthesis
• Ether lipid metabolism
• Ethylbenzene degradation
• Fatty acid biosynthesis
• Glycerophospholipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Histidine metabolism
• Limonene and pinene degradation
• Lipopolysaccharide biosynthesis
• Lysine degradation
• Phenylalanine metabolism
• Tyrosine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.-

Use Curated BLAST to search for 2.3.1.- or 2.7.7.58 or 6.2.1.71 or 6.3.2.14

Sequence Analysis Tools

See P10378 at UniProt or InterPro

Protein Sequence (536 amino acids)

>b0594 2,3-dihydroxybenzoate-AMP ligase component of enterobactin synthase multienzyme complex (NCBI) (Escherichia coli BW25113)
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGERQLSYRELNQAADN
LACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
PALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGEHNLQDAINHPAEDFTATPSP
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALIEGESRAQLA
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLRQWLASRASA