Protein Info for b0578 in Escherichia coli BW25113

Name: nfnB
Annotation: dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to NFSB_ECOLI: Oxygen-insensitive NAD(P)H nitroreductase (nfsB) from Escherichia coli (strain K12)

KEGG orthology group: K10679, nitroreductase / dihydropteridine reductase [EC: 1.-.-.- 1.5.1.34] (inferred from 100% identity to eco:b0578)

MetaCyc: 100% identical to NAD(P)H-dependent nitroreductase NfsB (Escherichia coli K-12 substr. MG1655)
1.7.1.-; NAD(P)H dehydrogenase (quinone). [EC: 1.6.5.2]; 6,7-dihydropteridine reductase. [EC: 1.6.5.2, 1.5.1.34]

Predicted SEED Role

"Oxygen-insensitive NAD(P)H nitroreductase (EC 1.-.-.-) / Dihydropteridine reductase (EC 1.5.1.34)" in subsystem Pterin biosynthesis (EC 1.-.-.-, EC 1.5.1.34)

MetaCyc Pathways

tetrahydropteridine recycling (2/2 steps found)
L-phenylalanine degradation V (1/3 steps found)
superpathway of photosynthetic hydrogen production (3/6 steps found)
vitamin K-epoxide cycle (1/4 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 1.6.5.2

Use Curated BLAST to search for 1.-.-.- or 1.5.1.34 or 1.6.5.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P38489 at UniProt or InterPro

Protein Sequence (217 amino acids)

>b0578 dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive (NCBI) (Escherichia coli BW25113)
MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQPWHFIVASTEEGKARV
AKSAAGNYVFNERKMLDASHVVVFCAKTAMDDVWLKLVVDQEDADGRFATPEAKAANDKG
RKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLDAVPIEGFDAAILDAEFGLKE
KGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV