Protein Info for b0570 in Escherichia coli BW25113

Name: cusS
Annotation: sensory histidine kinase in two-component regulatory system with CusR, senses copper ions (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 164 to 180 (17 residues), see Phobius details amino acids 187 to 207 (21 residues), see Phobius details PF21085: CusS" amino acids 8 to 180 (173 residues), 150.2 bits, see alignment E=1.4e-47 TIGR01386: heavy metal sensor kinase" amino acids 9 to 479 (471 residues), 523.7 bits, see alignment E=2.4e-161 PF00672: HAMP" amino acids 210 to 257 (48 residues), 41.6 bits, see alignment 3.2e-14 PF00512: HisKA" amino acids 262 to 326 (65 residues), 48.9 bits, see alignment E=1.4e-16 PF02518: HATPase_c" amino acids 371 to 479 (109 residues), 93.7 bits, see alignment E=2.4e-30 PF14501: HATPase_c_5" amino acids 376 to 475 (100 residues), 23.9 bits, see alignment E=8.5e-09

Best Hits

Swiss-Prot: 100% identical to CUSS_ECOLI: Sensor histidine kinase CusS (cusS) from Escherichia coli (strain K12)

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to eco:b0570)

MetaCyc: 100% identical to sensor histidine kinase CusS (Escherichia coli K-12 substr. MG1655)
Protein-histidine pros-kinase. [EC: 2.7.13.1]

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.1 or 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P77485 at UniProt or InterPro

Protein Sequence (480 amino acids)

>b0570 sensory histidine kinase in two-component regulatory system with CusR, senses copper ions (NCBI) (Escherichia coli BW25113)
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSQGKTVYHSPGAPDIREFTRDAIPDKDA
QGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVDGKPIYTLYIALSIDFHLHYI
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
RFVGDKCQVAGDPLMLRRALSNLLSNALRYTPTGETIVVRCQTVDHLVQVIVENPGTPIA
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSDARGTRFVITLPA