Protein Info for b0477 in Escherichia coli BW25113

Name: gsk
Annotation: inosine/guanosine kinase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to INGK_ECO57: Inosine-guanosine kinase (gsk) from Escherichia coli O157:H7

KEGG orthology group: K00892, inosine kinase [EC: 2.7.1.73] (inferred from 100% identity to eco:b0477)

MetaCyc: 100% identical to inosine/guanosine kinase (Escherichia coli K-12 substr. MG1655)
Inosine kinase. [EC: 2.7.1.73]; 2.7.1.73 [EC: 2.7.1.73]

Predicted SEED Role

"Inosine-guanosine kinase (EC 2.7.1.73)" in subsystem Purine conversions (EC 2.7.1.73)

MetaCyc Pathways

adenine and adenosine salvage V (3/3 steps found)
superpathway of guanine and guanosine salvage (3/3 steps found)
guanine and guanosine salvage III (1/1 steps found)

KEGG Metabolic Maps

Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.73

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AEW6 at UniProt or InterPro

Protein Sequence (434 amino acids)

>b0477 inosine/guanosine kinase (NCBI) (Escherichia coli BW25113)
MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLVDIEAKVDDEFIERYGL
SAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNYSVLADDRSVLLGVMCSNI
EIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESGERTFAISPGHMNQLRAESIP
EDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKKYNVPVVLTLGTKFVIAENPQWW
QQFLKDHVSILAMNEDEAEALTGESDPLLASDKALDWVDLVLCTAGPIGLYMAGFTEDEA
KRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPLRVYSHIAPYMGGPEKIMNTNGAGDGA
LAALLHDITANSYHRSNVPNSSKHKFTWLTYSSLAQVCKYANRVSYQVLNQHSPRLTRGL
PEREDSLEESYWDR