Protein Info for b0470 in Escherichia coli BW25113

Name: dnaX
Annotation: DNA polymerase III subunits gamma and tau (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 643 TIGR02397: DNA polymerase III, subunit gamma and tau" amino acids 3 to 356 (354 residues), 444.3 bits, see alignment E=1.8e-137 PF13177: DNA_pol3_delta2" amino acids 20 to 178 (159 residues), 147.8 bits, see alignment E=8.6e-47 PF07728: AAA_5" amino acids 42 to 147 (106 residues), 22.1 bits, see alignment E=3.8e-08 PF00004: AAA" amino acids 42 to 171 (130 residues), 42.7 bits, see alignment E=2.3e-14 PF12169: DNA_pol3_gamma3" amino acids 232 to 358 (127 residues), 101.9 bits, see alignment E=8.6e-33 PF12168: DNA_pol3_tau_4" amino acids 417 to 498 (82 residues), 86.1 bits, see alignment E=5.2e-28 PF12170: DNA_pol3_tau_5" amino acids 500 to 640 (141 residues), 194 bits, see alignment E=3.9e-61

Best Hits

Swiss-Prot: 100% identical to DPO3X_ECOLI: DNA polymerase III subunit tau (dnaX) from Escherichia coli (strain K12)

KEGG orthology group: K02343, DNA polymerase III subunit gamma/tau [EC: 2.7.7.7] (inferred from 100% identity to eco:b0470)

Predicted SEED Role

"DNA polymerase III subunits gamma and tau (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P06710 at UniProt or InterPro

Protein Sequence (643 amino acids)

>b0470 DNA polymerase III subunits gamma and tau (NCBI) (Escherichia coli BW25113)
MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFSGTRGVGKTSIARLLAK
GLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVEDTRDLLDNVQYAPARGRF
KVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQKLPVTILSRCLQFHLKALDV
EQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDALSLTDQAIASGDGQVSTQAVSAM
LGTLDDDQALSLVEAMVEANGERVMALINEAAARGIEWEALLVEMLGLLHRIAMVQLSPA
ALGNDMAAIELRMRELARTIPPTDIQLYYQTLLIGRKELPYAPDRRMGVEMTLLRALAFH
PRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQPQSAPQQAPTVPLPETTSQVLAARQQLQ
RVQGATKAKKSEPAAATRARPVNNAALERLASVTDRVQARPVPSALEKAPAKKEAYRWKA
TTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAAQVSQLSLPKLVEQVA
LNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPL
EWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI