Protein Info for b0465 in Escherichia coli BW25113

Name: kefA
Annotation: fused conserved protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1120 signal peptide" amino acids 1 to 36 (36 residues), see Phobius details transmembrane" amino acids 499 to 522 (24 residues), see Phobius details amino acids 557 to 579 (23 residues), see Phobius details amino acids 587 to 606 (20 residues), see Phobius details amino acids 630 to 647 (18 residues), see Phobius details amino acids 658 to 679 (22 residues), see Phobius details amino acids 691 to 716 (26 residues), see Phobius details amino acids 728 to 749 (22 residues), see Phobius details amino acids 795 to 820 (26 residues), see Phobius details amino acids 840 to 860 (21 residues), see Phobius details amino acids 881 to 903 (23 residues), see Phobius details amino acids 914 to 941 (28 residues), see Phobius details PF12795: MscS_porin" amino acids 50 to 289 (240 residues), 211.1 bits, see alignment E=4.8e-66 PF12794: MscS_TM" amino acids 509 to 826 (318 residues), 331.8 bits, see alignment E=1e-102 PF21088: MS_channel_1st" amino acids 887 to 928 (42 residues), 43.4 bits, see alignment (E = 6.4e-15) PF00924: MS_channel_2nd" amino acids 930 to 995 (66 residues), 85.6 bits, see alignment 5e-28 PF21082: MS_channel_3rd" amino acids 1004 to 1086 (83 residues), 75.9 bits, see alignment 6.8e-25

Best Hits

Swiss-Prot: 100% identical to MSCK_ECOLI: Mechanosensitive channel MscK (mscK) from Escherichia coli (strain K12)

KEGG orthology group: K05802, potassium efflux system protein KefA (inferred from 100% identity to ect:ECIAI39_0206)

MetaCyc: 100% identical to potassium dependent, small conductance mechanosensitive channel (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-86

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P77338 at UniProt or InterPro

Protein Sequence (1120 amino acids)

>b0465 fused conserved protein (NCBI) (Escherichia coli BW25113)
MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTKADLQAQLDSLNKQKDL
SAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKMRQATAALTALSDVDNDEE
TRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVSLQTQPERVQNAMYNASQQLQ
QIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQRKSLEGNTVLQDTLQKQRDYVT
ANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPDEAARIQANPLVKQELEINQQLSQR
LITATENGNQLMQQNIKVKNWLERALQSERNIKEQIAVLKGSLLLSRILYQQQQTLPSAD
ELENMTNRIADLRLEQFEVNQQRDALFQSDAFVNKLEEGHTNEVNSEVHDALLQVVDMRR
ELLDQLNKQLGNQLMMAINLQINQQQLMSVSKNLKSILTQQIFWVNSNRPMDWDWIKAFP
QSLKDEFKSMKITVNWQKAWPAVFIAFLAGLPLLLIAGLIHWRLGWLKAYQQKLASAVGS
LRNDSQLNTPKAILIDLIRALPVCLIILAVGLILLTMQLNISELLWSFSKKLAIFWLVFG
LCWKVLEKNGVAVRHFGMPEQQTSHWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLG
QAMIFFNLLLIAFLVWPMCRESWRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLR
LAGRWIETVYLVIIWNLLYQTVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPT
IALEQVNQQTLRITMLLMFALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKN
VTMGSLLFAIIASMVAWALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMT
VFGSLGVSWDKLQWLAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGT
VSKIRIRATTITDFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRK
VLLKAATEHPRVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCR
ENDINIAFNQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG