Protein Info for b0454 in Escherichia coli BW25113

Name: ybaZ
Annotation: predicted methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 129 TIGR00589: methylated-DNA--[protein]-cysteine S-methyltransferase" amino acids 32 to 102 (71 residues), 52.8 bits, see alignment E=1.6e-18 PF01035: DNA_binding_1" amino acids 33 to 112 (80 residues), 94 bits, see alignment E=2.1e-31

Best Hits

Swiss-Prot: 100% identical to ATL_SHIFL: DNA base-flipping protein (atl) from Shigella flexneri

KEGG orthology group: K07443, methylated-DNA-protein-cysteine methyltransferase related protein (inferred from 100% identity to eco:b0454)

Predicted SEED Role

"methylated-DNA--protein-cysteine methyltransferase-related protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AFP2 at UniProt or InterPro

Protein Sequence (129 amino acids)

>b0454 predicted methyltransferase (NCBI) (Escherichia coli BW25113)
MLVSCAMRLHSGVFPDYAEKLPQEEKMEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAG
SPRAARQVGGVLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQI
DLQRYRWNY