Protein Info for b0451 in Escherichia coli BW25113

Name: amtB
Annotation: ammonium transporter (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 428 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details transmembrane" amino acids 32 to 55 (24 residues), see Phobius details amino acids 66 to 87 (22 residues), see Phobius details amino acids 119 to 142 (24 residues), see Phobius details amino acids 149 to 171 (23 residues), see Phobius details amino acids 183 to 205 (23 residues), see Phobius details amino acids 218 to 237 (20 residues), see Phobius details amino acids 248 to 269 (22 residues), see Phobius details amino acids 280 to 297 (18 residues), see Phobius details amino acids 301 to 324 (24 residues), see Phobius details amino acids 336 to 359 (24 residues), see Phobius details amino acids 375 to 396 (22 residues), see Phobius details TIGR00836: ammonium transporter" amino acids 31 to 426 (396 residues), 464.2 bits, see alignment E=1.8e-143 PF00909: Ammonium_transp" amino acids 33 to 426 (394 residues), 444.5 bits, see alignment E=1.6e-137

Best Hits

Swiss-Prot: 100% identical to AMTB_ECO57: Ammonia channel (amtB) from Escherichia coli O157:H7

KEGG orthology group: K03320, ammonium transporter, Amt family (inferred from 100% identity to eco:b0451)

MetaCyc: 100% identical to ammonium transporter (Escherichia coli K-12 substr. MG1655)
RXN-9615

Predicted SEED Role

"Ammonium transporter" in subsystem Ammonia assimilation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P69681 at UniProt or InterPro

Protein Sequence (428 amino acids)

>b0451 ammonium transporter (NCBI) (Escherichia coli BW25113)
MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLFMTIPGIALFYGGLIRG
KNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINWLMLKNIELTAVMGSIYQY
IHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTLSYIPIAHMVWGGGLLASHGA
LDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPHNLPMVFTGTAILYIGWFGFNAG
SAGTANEIAALAFVNTVVATAAAILGWIFGEWALRGKPSLLGACSGAIAGLVGVTPACGY
IGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPCDVFGVHGVCGIVGCIMTGIFAASSLG
GVGFAEGVTMGHQLLVQLESIAITIVWSGVVAFIGYKLADLTVGLRVPEEQEREGLDVNS
HGENAYNA