Protein Info for b0398 in Escherichia coli BW25113

Name: sbcD
Annotation: exonuclease, dsDNA, ATP-dependent (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 PF00149: Metallophos" amino acids 1 to 225 (225 residues), 61.7 bits, see alignment E=2e-20 TIGR00619: exonuclease SbcCD, D subunit" amino acids 1 to 254 (254 residues), 318.6 bits, see alignment E=1.8e-99 PF12320: SbcD_C" amino acids 276 to 373 (98 residues), 64.6 bits, see alignment E=1.4e-21

Best Hits

Swiss-Prot: 100% identical to SBCD_ECO57: Nuclease SbcCD subunit D (sbcD) from Escherichia coli O157:H7

KEGG orthology group: K03547, exonuclease SbcD (inferred from 100% identity to eco:b0398)

MetaCyc: 100% identical to ATP-dependent structure-specific DNA nuclease - SbcD subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Exonuclease SbcD" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AG76 at UniProt or InterPro

Protein Sequence (400 amino acids)

>b0398 exonuclease, dsDNA, ATP-dependent (NCBI) (Escherichia coli BW25113)
MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAIIVAGDVFDTGSPPSYAR
TLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNTTVVASAGHAPQILPRRDG
TPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDYYQQHYADACKLRGDQPLPII
ATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYIALGHIHRAQIIGGMEHVRYCGS
PIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVTQPMAVLKGDLASITAQLEQWRDVS
QEPPVWLDIEITTDEYLHDIQRKIQALTESLPVEVLLVRRSREQRERVLASQQRETLSEL
SVEEVFNRRLALEELDESQQQRLQHLFTTTLHTLAGEHEA