Protein Info for b0394 in Escherichia coli BW25113

Name: yajF
Annotation: possible NAGC-like transcriptional regulator (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00480: ROK
  • amino acids 3 to 301 (299 residues), 339.6 bits, E=1.8e-105
• PF02685: Glucokinase
  • amino acids 6 to 242 (237 residues), 39.3 bits, E=4.2e-14

Best Hits

Swiss-Prot: 100% identical to MAK_ECOLI: Fructokinase (mak) from Escherichia coli (strain K12)

KEGG orthology group: K00847, fructokinase [EC: 2.7.1.4] (inferred from 100% identity to eco:b0394)

MetaCyc: 100% identical to fructokinase (Escherichia coli K-12 substr. MG1655)
Mannokinase. [EC: 2.7.1.7]; Fructokinase. [EC: 2.7.1.7, 2.7.1.4]

Predicted SEED Role

"Glucokinase, ROK family (EC 2.7.1.2)" in subsystem Beta-Glucoside Metabolism (EC 2.7.1.2)

MetaCyc Pathways

• homolactic fermentation (12/12 steps found)
• superpathway of anaerobic sucrose degradation (17/19 steps found)
• glycolysis III (from glucose) (11/11 steps found)
• heterolactic fermentation (16/18 steps found)
• glycogen degradation I (8/8 steps found)
• Bifidobacterium shunt (13/15 steps found)
• glucose and glucose-1-phosphate degradation (5/5 steps found)
• sucrose degradation IV (sucrose phosphorylase) (4/4 steps found)
• D-sorbitol degradation I (3/3 steps found)
• trehalose degradation IV (3/3 steps found)
• D-mannose degradation II (2/2 steps found)
• trehalose degradation I (low osmolarity) (2/2 steps found)
• trehalose degradation II (cytosolic) (2/2 steps found)
• 1,3-propanediol biosynthesis (engineered) (7/9 steps found)
• mannitol cycle (4/5 steps found)
• sucrose degradation II (sucrose synthase) (4/5 steps found)
• sucrose biosynthesis II (6/8 steps found)
• mannitol degradation II (3/4 steps found)
• sucrose degradation III (sucrose invertase) (3/4 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• GDP-α-D-glucose biosynthesis (2/3 steps found)
• trehalose degradation V (2/3 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• glycogen degradation II (4/6 steps found)
• chitin biosynthesis (6/9 steps found)
• 1,5-anhydrofructose degradation (3/5 steps found)
• sucrose degradation I (sucrose phosphotransferase) (1/3 steps found)
• CMP-2-keto-3-deoxy-D-glycero-D-galacto-nononate biosynthesis (1/4 steps found)
• sucrose degradation VII (sucrose 3-dehydrogenase) (1/4 steps found)
• superpathway of CMP-sialic acids biosynthesis (3/15 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism
• Streptomycin biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.2, 2.7.1.4

Use Curated BLAST to search for 2.7.1.2 or 2.7.1.4 or 2.7.1.7

Sequence Analysis Tools

See P23917 at UniProt or InterPro

Protein Sequence (302 amino acids)

>b0394 possible NAGC-like transcriptional regulator (VIMSS) (Escherichia coli BW25113)
MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIATLVDMAEQATGQRGTV
GMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRLANDANCLAVSEAVDGAAA
GAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPLPWMDEDELRYREEVPCYCGK
QGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESDPVAELALRRYELRLAKSLAHVV
NILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGECETPVRKAKHGDSSGVRGAAWLWP
QE