Protein Info for b0349 in Escherichia coli BW25113

Name: mhpC
Annotation: 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12146: Hydrolase_4
  • amino acids 40 to 272 (233 residues), 46 bits, E=6.1e-16
• PF00561: Abhydrolase_1
  • amino acids 42 to 279 (238 residues), 89.4 bits, E=4.6e-29
• PF12697: Abhydrolase_6
  • amino acids 43 to 284 (242 residues), 87.2 bits, E=3.9e-28

Best Hits

Swiss-Prot: 100% identical to MHPC_ECOLI: 2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase (mhpC) from Escherichia coli (strain K12)

KEGG orthology group: K05714, 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase [EC: 3.7.1.-] (inferred from 100% identity to eco:b0349)

MetaCyc: 100% identical to 2-hydroxy-6-ketonona-2,4-dienedioate hydrolase (Escherichia coli K-12 substr. MG1655)
MHPCHYDROL-RXN [EC: 3.7.1.14]; 3.7.1.14 [EC: 3.7.1.14]

Predicted SEED Role

"2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (EC 3.7.1.-)" in subsystem Cinnamic Acid Degradation (EC 3.7.1.-)

MetaCyc Pathways

• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation (8/8 steps found)
• 3-phenylpropanoate and 3-(3-hydroxyphenyl)propanoate degradation to 2-hydroxypentadienoate (5/5 steps found)
• cinnamate and 3-hydroxycinnamate degradation to 2-hydroxypentadienoate (5/5 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Ascorbate and aldarate metabolism
• Benzoate degradation via CoA ligation
• Benzoate degradation via hydroxylation
• Biphenyl degradation
• Caprolactam degradation
• Ethylbenzene degradation
• Inositol phosphate metabolism
• Limonene and pinene degradation
• Nicotinate and nicotinamide metabolism
• Phenylalanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.7.1.- or 3.7.1.14

Sequence Analysis Tools

See P77044 at UniProt or InterPro

Protein Sequence (293 amino acids)

>b0349 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS) (Escherichia coli BW25113)
MQEKMMSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGSGPGATGWANFS
RNIDPLVEAGYRVILLDCPGWGKSDSVVNSGSRSDLNARILKSVVDQLDIAKIHLLGNSM
GGHSSVAFTLKWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQPTIENLKLMMD
IFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPRLAEIKAQTLIVW
GRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLVLNFLARP