Protein Info for b0269 in Escherichia coli BW25113
Name: yagF
Annotation: CP4-6 prophage; predicted dehydratase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to YAGF_ECOLI: D-xylonate dehydratase YagF (yagF) from Escherichia coli (strain K12)
KEGG orthology group: None (inferred from 100% identity to eco:b0269)MetaCyc: 100% identical to CP4-6 prophage; D-xylonate dehydratase (Escherichia coli K-12 substr. MG1655)
Xylonate dehydratase. [EC: 4.2.1.82]
Predicted SEED Role
"Dihydroxy-acid dehydratase (EC 4.2.1.9)" in subsystem Branched-Chain Amino Acid Biosynthesis (EC 4.2.1.9)
MetaCyc Pathways
- superpathway of branched chain amino acid biosynthesis (17/17 steps found)
- superpathway of L-isoleucine biosynthesis I (13/13 steps found)
- superpathway of L-threonine metabolism (16/18 steps found)
- L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
- L-valine biosynthesis (4/4 steps found)
- pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
- D-xylose degradation to ethylene glycol (engineered) (3/4 steps found)
- L-isoleucine biosynthesis IV (4/6 steps found)
- L-isoleucine biosynthesis III (4/7 steps found)
- L-isoleucine biosynthesis II (4/8 steps found)
- D-xylose degradation IV (3/7 steps found)
- D-xylose degradation III (1/5 steps found)
- D-xylose degradation V (1/5 steps found)
- D-xylose degradation VI (1/5 steps found)
- superpathway of pentose and pentitol degradation (18/42 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Pantothenate and CoA biosynthesis
- Pentose and glucuronate interconversions
- Valine, leucine and isoleucine biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 4.2.1.9
Use Curated BLAST to search for 4.2.1.82 or 4.2.1.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See P77596 at UniProt or InterPro
Protein Sequence (655 amino acids)
>b0269 CP4-6 prophage; predicted dehydratase (NCBI) (Escherichia coli BW25113) MTIEKIFTPQDDAFYAVITHAAGPQGALPLTPQMLMESPSGNLFGMTQNAGMGWDANKLT GKEVLIIGTQGGIRAGDGRPIALGYHTGHWEIGMQMQAAAKEITRNGGIPFAAFVSDPCD GRSQGTHGMFDSLPYRNDAAIVFRRLIRSLPTRRAVIGVATCDKGLPATMIALAAMHDLP TILVPGGATLPPTVGEDAGKVQTIGARFANHELSLQEAAELGCRACASPGGGCQFLGTAG TSQVVAEALGLALPHSALAPSGQAVWLEIARQSARAVSELDSRGITTRDILSDKAIENAM VIHAAFGGSTNLLLHIPAIAHAAGCTIPDVEHWTRINRKVPRLVSVLPNGPDYHPTVRAF LAGGVPEVMLHLRDLGLLHLDAMTVTGQTVGENLEWWQASERRARFRQCLREQDGVEPDD VILPPEKAKAKGLTSTVCFPTGNIAPEGSVIKATAIDPSVVGEDGVYHHTGRVRVFVSEA QAIKAIKREEIVQGDIMVVIGGGPSGTGMEETYQLTSALKHISWGKTVSLITDARFSGVS TGACFGHVSPEALAGGPIGKLRDNDIIEIAVDRLTLTGSVNFIGTADNPLTPEEGARELA RRQTHPDLHAHDFLPDDTRLWAALQSVSGGTWKGCIYDTDKIIEVINAGKKALGI