Protein Info for b0224 in Escherichia coli BW25113

Name: yafK
Annotation: hypothetical protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 246 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF03734: YkuD" amino acids 45 to 159 (115 residues), 32.9 bits, see alignment E=4.7e-12

Best Hits

Swiss-Prot: 100% identical to YAFK_ECO57: Putative L,D-transpeptidase YafK (yafK) from Escherichia coli O157:H7

KEGG orthology group: None (inferred from 100% identity to eco:b0224)

MetaCyc: 100% identical to peptidoglycan meso-diaminopimelic acid protein amidase A (Escherichia coli K-12 substr. MG1655)
3.4.-.-; 3.4.-.-

Predicted SEED Role

"probable exported protein STY0357"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P0AA99 at UniProt or InterPro

Protein Sequence (246 amino acids)

>b0224 hypothetical protein (NCBI) (Escherichia coli BW25113)
MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPVYIQIFKEERTLDLYVK
MGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQLKPDSRYYKAINIGFPNA
YDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTGALVFGQPSVQVSIYPFRMTD
ANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVNGRYVVSKPLSHEVVQPQLASNY
TLPEAK