Protein Info for b0167 in Escherichia coli BW25113

Name: glnD
Annotation: PII uridylyl-transferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 890 PF03445: DUF294" amino acids 43 to 125 (83 residues), 24.2 bits, see alignment E=6.7e-09 TIGR01693: protein-P-II uridylyltransferase" amino acids 51 to 884 (834 residues), 1002.8 bits, see alignment E=7.5e-306 PF01909: NTP_transf_2" amino acids 85 to 143 (59 residues), 28.7 bits, see alignment 3.7e-10 PF08335: GlnD_UR_UTase" amino acids 193 to 331 (139 residues), 156.4 bits, see alignment E=1.2e-49 PF01966: HD" amino acids 468 to 601 (134 residues), 47.9 bits, see alignment E=4e-16 PF01842: ACT" amino acids 709 to 762 (54 residues), 34.1 bits, see alignment 4.6e-12 amino acids 817 to 874 (58 residues), 36.9 bits, see alignment 6.4e-13

Best Hits

Swiss-Prot: 100% identical to GLND_ECOLI: Bifunctional uridylyltransferase/uridylyl-removing enzyme (glnD) from Escherichia coli (strain K12)

KEGG orthology group: K00990, [protein-PII] uridylyltransferase [EC: 2.7.7.59] (inferred from 100% identity to ecj:JW0162)

Predicted SEED Role

"[Protein-PII] uridylyltransferase (EC 2.7.7.59)" in subsystem Ammonia assimilation (EC 2.7.7.59)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.59

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P27249 at UniProt or InterPro

Protein Sequence (890 amino acids)

>b0167 PII uridylyl-transferase (NCBI) (Escherichia coli BW25113)
MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQRWLGDAFDNGISAEQL
IEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHPLSDVDLLILSRKKLPDDQ
AQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVATNLIESRLLIGDVALFLELQK
HIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPDIKSSPGGLRDIHTLQWVARRHF
GATSLDEMVGFGFLTSAERAELNECLHILWRIRFALHLVVSRYDNRLLFDRQLSVAQRLN
YSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLFDEAILALPADEKPRPIDDEFQLRGTL
IDLRDETLFMRQPEAILRMFYTMVHNSAITGIYSTTLRQLRHARRHLQQPLCNIPEARKL
FLSILRHPGAVRRGLLPMHRHSVLGAYMPQWSHIVGQMQFDLFHAYTVDEHTIRVMLKLE
SFASEETRQRHPLCVDVWPRLPSTELIFIAALFHDIAKGRGGDHSILGAQDVVHFAELHG
LNSRETQLVAWLVRQHLLMSVTAQRRDIQDPEVIKQFAEEVQTENRLRYLVCLTVADICA
TNETLWNSWKQSLLRELYFATEKQLRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEAL
HQIWSRCRANYFVRHSPNQLAWHARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYL
FAAVCAELDRRNLSVHDAQIFTTRDGMAMDTFIVLEPDGNPLSADRHEVIRFGLEQVLTQ
SSWQPPQPRRQPAKLRHFTVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGI
SLHGARITTIGERVEDLFIIATADRRALNNELQQEVHQRLTEALNPNDKG