Protein Info for b0148 in Escherichia coli BW25113

Name: hrpB
Annotation: helicase, ATP-dependent (VIMSS)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 809 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details TIGR01970: ATP-dependent helicase HrpB" amino acids 4 to 808 (805 residues), 1276.2 bits, see alignment E=0 PF00270: DEAD" amino acids 21 to 164 (144 residues), 45.9 bits, see alignment E=1.1e-15 PF00271: Helicase_C" amino acids 208 to 327 (120 residues), 37.9 bits, see alignment E=3.9e-13 PF24473: CON_HrpB" amino acids 566 to 600 (35 residues), 51.9 bits, see alignment (E = 1e-17) PF08482: HrpB_C" amino acids 664 to 796 (133 residues), 182.5 bits, see alignment E=8.7e-58

Best Hits

Swiss-Prot: 100% identical to HRPB_ECOLI: ATP-dependent RNA helicase HrpB (hrpB) from Escherichia coli (strain K12)

KEGG orthology group: K03579, ATP-dependent helicase HrpB [EC: 3.6.4.13] (inferred from 100% identity to ecj:JW0144)

Predicted SEED Role

"ATP-dependent helicase HrpB"

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P37024 at UniProt or InterPro

Protein Sequence (809 amino acids)

>b0148 helicase, ATP-dependent (VIMSS) (Escherichia coli BW25113)
MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAHPGINGKIILLEPRRLA
ARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEGVLTRMIQRDPELSGVGLV
ILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLDNDRLQQMLPEAPVVISEGRS
FPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLFLPGVGEIQRVQEQLASRIGSDV
LLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAETSLTIEGIRLVVDCAQERVARFDP
RTGLTRLITQRVSQASMTQRAGRAGRLEPGISLHLIAKEQAERAAAQSEPEILQSDLSGL
LMELLQWGCSDPAQMSWLDQPPVVNLLAAKRLLQMLGALEGERLSAQGQKMAALGNDPRL
AAMLVSAKNDDEAATAAKIAAILEEPPRMGNSDLGVAFSRNQPAWQQRSQQLLKRLNVRG
GEADSSLIAPLLAGAFADRIARRRGQDGRYQLANGMGAMLDANDALSRHEWLIAPLLLQG
SASPDARILLALLVDIDELVQRCPQLVQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPL
AKPSEDELHQAMLNGIRDKGLSVLNWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAA
LETWLLPHMTGVHSLRGLKSLDIYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYH
EDNPPALAVRMQEMFGEATNPTIAQGRVPLVLELLSPAQRPLQITRDLSDFWKGAYREVQ
KEMKGRYPKHVWPDDPANTAPTRRTKKYS