Protein Info for b0129 in Escherichia coli BW25113

Name: yadI
Annotation: predicted PTS Enzyme IIA (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03610: EIIA-man
  • amino acids 3 to 116 (114 residues), 107.2 bits, E=2.9e-35

Best Hits

Swiss-Prot: 100% identical to YADI_ECOLI: Putative phosphotransferase enzyme IIA component YadI (yadI) from Escherichia coli (strain K12)

KEGG orthology group: K02821, PTS system, ascorbate-specific IIA component [EC: 2.7.1.69] (inferred from 100% identity to eco:b0129)

MetaCyc: 100% identical to putative PTS enzyme IIA component YadI (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Putative PTS system IIA component yadI (EC 2.7.1.69)" (EC 2.7.1.69)

KEGG Metabolic Maps

• Aminosugars metabolism
• Ascorbate and aldarate metabolism
• Fructose and mannose metabolism
• Galactose metabolism
• Glycolysis / Gluconeogenesis
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

See P36881 at UniProt or InterPro

Protein Sequence (146 amino acids)

>b0129 predicted PTS Enzyme IIA (NCBI) (Escherichia coli BW25113)
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEG
VIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELG
APEVSSLWHQQQKNPPFVLKHNLYEY