Protein Info for b0123 in Escherichia coli BW25113

Name: cueO
Annotation: multicopper oxidase (laccase) (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF07732: Cu-oxidase_3" amino acids 54 to 165 (112 residues), 109.7 bits, see alignment E=1.4e-35 PF00394: Cu-oxidase" amino acids 227 to 293 (67 residues), 21.9 bits, see alignment E=2.4e-08 PF07731: Cu-oxidase_2" amino acids 399 to 516 (118 residues), 82.8 bits, see alignment E=3e-27

Best Hits

Swiss-Prot: 100% identical to CUEO_ECOLI: Blue copper oxidase CueO (cueO) from Escherichia coli (strain K12)

KEGG orthology group: K14588, blue copper oxidase (inferred from 100% identity to eco:b0123)

MetaCyc: 100% identical to multicopper oxidase CueO (Escherichia coli K-12 substr. MG1655)
RXN0-2945 [EC: 1.16.3.4]; Ferroxidase. [EC: 1.16.3.4, 1.16.3.1]; Laccase. [EC: 1.16.3.4, 1.16.3.1, 1.10.3.2]; RXN0-2943 [EC: 1.16.3.4, 1.16.3.1, 1.10.3.2]

Predicted SEED Role

"Blue copper oxidase CueO precursor" in subsystem Copper homeostasis

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.16.3.1

Use Curated BLAST to search for 1.10.3.2 or 1.16.3.1 or 1.16.3.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P36649 at UniProt or InterPro

Protein Sequence (516 amino acids)

>b0123 multicopper oxidase (laccase) (NCBI) (Escherichia coli BW25113)
MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDARNRIQLTIGAGQSTFG
GKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHWHGLEVPGEVDGGPQGIIP
PGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLVVIEDDEILKLMLPKQWGIDD
VPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNGAIYPQHAAPRGWLRLRLLNGCN
ARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVLMGERFEVLVEVNDNKPFDLVTLPV
SQMGMAIAPFDKPHPVMRIQPIAISASGALPDTLSSLPALPSLEGLTVRKLQLSMDPMLD
MMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGNMNHMNHGGKFDFHHANKINGQAFDMNKP
MFAAAKGQYERWVISGVGDMMLHPFHIHGTQFRILSENGKPPAAHRAGWKDTVKVEGNVS
EVLVKFNHDAPKEHAYMAHCHLLEHEDTGMMLGFTV