Protein Info for b0049 in Escherichia coli BW25113

Name: apaH
Annotation: diadenosinetetraphosphatase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 280 TIGR00668: bis(5'-nucleosyl)-tetraphosphatase (symmetrical)" amino acids 1 to 278 (278 residues), 557 bits, see alignment E=4.2e-172 PF00149: Metallophos" amino acids 3 to 126 (124 residues), 61.3 bits, see alignment E=9e-21

Best Hits

Swiss-Prot: 100% identical to APAH_SHISS: Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (apaH) from Shigella sonnei (strain Ss046)

KEGG orthology group: K01525, bis(5'-nucleosyl)-tetraphosphatase (symmetrical) [EC: 3.6.1.41] (inferred from 100% identity to eco:b0049)

MetaCyc: 100% identical to diadenosine tetraphosphatase (Escherichia coli K-12 substr. MG1655)
Bis(5'-nucleosyl)-tetraphosphatase (symmetrical). [EC: 3.6.1.41]; Guanosine-5'-triphosphate,3'-diphosphate diphosphatase. [EC: 3.6.1.41, 3.6.1.40]

Predicted SEED Role

"Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)" (EC 3.6.1.41)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.40

Use Curated BLAST to search for 3.6.1.40 or 3.6.1.41

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See P05637 at UniProt or InterPro

Protein Sequence (280 amino acids)

>b0049 diadenosinetetraphosphatase (NCBI) (Escherichia coli BW25113)
MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPGSLDVLRYVKSLGDSVR
LVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWLRRQPLLQIDEEKKLVMAH
AGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMPNNWSPELRGLGRLRFITNAF
TRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVAEEYSIAFGHWASLEGKGTPEGI
YALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGEAAAS