Top cofit genes for b0223 from Escherichia coli BW25113

yafJ: predicted amidotransfease (NCBI)
SEED: Predicted glutamine amidotransferase
KEGG: glutamine amidotransferase

Rank	Hit	Name	Description	Conserved?	Cofitness
1	b4018	iclR	repressor of aceBA operon (VIMSS)	no	0.63
2	b2414	cysK	cysteine synthase A, O-acetylserine sulfhydrolase A subunit (NCBI)	0.59	0.62
3	b2752	cysD	sulfate adenylyltransferase subunit 2 (NCBI)	0.62	0.61
4	b3008	metC	cystathionine beta-lyase (NCBI)	0.62	0.61
5	b2020	hisD	histidinol dehydrogenase (NCBI)	0.62	0.60
6	b2600	tyrA	fused chorismate mutase T/prephenate dehydrogenase (NCBI)	0.57	0.60
7	b2022	hisB	imidazoleglycerolphosphate dehydratase and histidinol-phosphate phosphatase (VIMSS)	0.57	0.60
8	b3774	ilvC	ketol-acid reductoisomerase (NCBI)	0.63	0.60
9	b2763	cysI	sulfite reductase, beta subunit, NAD(P)-binding, heme-binding (NCBI)	0.62	0.60
10	b3368	cysG	fused siroheme synthase 1,3-dimethyluroporphyriongen III dehydrogenase and siroheme ferrochelatase/uroporphyrinogen methyltransferase (NCBI)	0.63	0.60
11	b3771	ilvD	dihydroxy-acid dehydratase (NCBI)	0.61	0.60
12	b2021	hisC	histidinol-phosphate aminotransferase (NCBI)	0.69	0.59
13	b2751	cysN	sulfate adenylyltransferase subunit 1 (NCBI)	0.68	0.59
14	b3770	ilvE	branched-chain amino acid aminotransferase (NCBI)	0.60	0.59
15	b2019	hisG	ATP phosphoribosyltransferase (NCBI)	0.54	0.59
16	b3941	metF	5,10-methylenetetrahydrofolate reductase (NCBI)	0.63	0.59
17	b2764	cysJ	sulfite reductase, alpha subunit, flavoprotein (NCBI)	0.61	0.59
18	b0002	thrA	bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI)	0.62	0.59
19	b0003	thrB	homoserine kinase (NCBI)	0.57	0.59
20	b0242	proB	gamma-glutamyl kinase (NCBI)	0.63	0.59

Or look for negative cofitness