Experiment: Parabanic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_116 and Pf6N2E2_117 overlap by 8 nucleotides Pf6N2E2_117 and Pf6N2E2_118 overlap by 4 nucleotides Pf6N2E2_118 and Pf6N2E2_119 overlap by 4 nucleotides
Pf6N2E2_116 - Glycosyltransferase, at 162,648 to 163,784
_116
Pf6N2E2_117 - Glycosyl transferase, group 2 family protein, at 163,777 to 164,736
_117
Pf6N2E2_118 - FIG00961753: hypothetical protein, at 164,733 to 166,103
_118
Pf6N2E2_119 - Glycosyltransferase, at 166,100 to 167,203
_119
Position (kb)
164
165
166
167 Strain fitness (log2 ratio)
-1
0
1
2 at 163.735 kb on + strand at 163.741 kb on + strand at 163.742 kb on - strand at 163.768 kb on + strand at 163.769 kb on - strand at 164.705 kb on + strand at 164.705 kb on + strand at 164.947 kb on + strand, within Pf6N2E2_118 at 164.947 kb on + strand, within Pf6N2E2_118 at 164.948 kb on - strand, within Pf6N2E2_118 at 165.019 kb on + strand, within Pf6N2E2_118 at 165.019 kb on + strand, within Pf6N2E2_118 at 165.019 kb on + strand, within Pf6N2E2_118 at 165.020 kb on - strand, within Pf6N2E2_118 at 165.097 kb on + strand, within Pf6N2E2_118 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.172 kb on + strand, within Pf6N2E2_118 at 165.173 kb on - strand, within Pf6N2E2_118 at 165.364 kb on + strand, within Pf6N2E2_118 at 165.384 kb on - strand, within Pf6N2E2_118 at 165.474 kb on + strand, within Pf6N2E2_118 at 165.475 kb on - strand, within Pf6N2E2_118 at 165.589 kb on + strand, within Pf6N2E2_118 at 165.778 kb on + strand, within Pf6N2E2_118 at 165.812 kb on + strand, within Pf6N2E2_118 at 165.813 kb on - strand, within Pf6N2E2_118 at 165.982 kb on + strand at 166.131 kb on + strand at 166.131 kb on + strand at 166.132 kb on - strand at 166.294 kb on + strand, within Pf6N2E2_119 at 166.310 kb on + strand, within Pf6N2E2_119 at 166.310 kb on + strand, within Pf6N2E2_119 at 166.311 kb on - strand, within Pf6N2E2_119 at 166.311 kb on - strand, within Pf6N2E2_119 at 166.311 kb on - strand, within Pf6N2E2_119 at 166.455 kb on + strand, within Pf6N2E2_119 at 166.822 kb on + strand, within Pf6N2E2_119 at 166.823 kb on - strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.824 kb on + strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119 at 166.825 kb on - strand, within Pf6N2E2_119
Per-strain Table
Position Strand Gene LocusTag Fraction Parabanic Acid (N) remove 163,735 + +1.1 163,741 + -0.1 163,742 - +0.8 163,768 + +0.8 163,769 - -0.5 164,705 + -0.9 164,705 + +0.6 164,947 + Pf6N2E2_118 0.16 +1.8 164,947 + Pf6N2E2_118 0.16 +0.2 164,948 - Pf6N2E2_118 0.16 +1.4 165,019 + Pf6N2E2_118 0.21 +0.7 165,019 + Pf6N2E2_118 0.21 +1.2 165,019 + Pf6N2E2_118 0.21 +0.1 165,020 - Pf6N2E2_118 0.21 +1.0 165,097 + Pf6N2E2_118 0.27 +0.8 165,172 + Pf6N2E2_118 0.32 +0.1 165,172 + Pf6N2E2_118 0.32 +1.0 165,172 + Pf6N2E2_118 0.32 +0.0 165,172 + Pf6N2E2_118 0.32 -0.6 165,173 - Pf6N2E2_118 0.32 -0.2 165,364 + Pf6N2E2_118 0.46 +1.2 165,384 - Pf6N2E2_118 0.47 -0.2 165,474 + Pf6N2E2_118 0.54 +1.2 165,475 - Pf6N2E2_118 0.54 +1.3 165,589 + Pf6N2E2_118 0.62 +0.7 165,778 + Pf6N2E2_118 0.76 +0.7 165,812 + Pf6N2E2_118 0.79 +0.4 165,813 - Pf6N2E2_118 0.79 +0.8 165,982 + +0.8 166,131 + -0.2 166,131 + -0.1 166,132 - +0.3 166,294 + Pf6N2E2_119 0.18 +0.8 166,310 + Pf6N2E2_119 0.19 +1.1 166,310 + Pf6N2E2_119 0.19 +0.4 166,311 - Pf6N2E2_119 0.19 +0.1 166,311 - Pf6N2E2_119 0.19 -0.8 166,311 - Pf6N2E2_119 0.19 +0.4 166,455 + Pf6N2E2_119 0.32 +0.7 166,822 + Pf6N2E2_119 0.65 +0.4 166,823 - Pf6N2E2_119 0.65 +2.0 166,824 + Pf6N2E2_119 0.66 +0.1 166,824 + Pf6N2E2_119 0.66 +0.1 166,824 + Pf6N2E2_119 0.66 +0.9 166,824 + Pf6N2E2_119 0.66 +1.0 166,825 - Pf6N2E2_119 0.66 +0.2 166,825 - Pf6N2E2_119 0.66 -0.5 166,825 - Pf6N2E2_119 0.66 -0.0 166,825 - Pf6N2E2_119 0.66 -0.1 166,825 - Pf6N2E2_119 0.66 +1.2 166,825 - Pf6N2E2_119 0.66 -0.1
Or see this region's nucleotide sequence