Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6124 and Pf6N2E2_3462 are separated by 634 nucleotides Pf6N2E2_3462 and Pf6N2E2_3463 are separated by 71 nucleotides
Pf6N2E2_6124 - Small Subunit Ribosomal RNA; ssuRNA; SSU rRNA, at 4,040,400 to 4,041,928
_6124
Pf6N2E2_3462 - Methylmalonate-semialdehyde dehydrogenase (EC 1.2.1.27), at 4,042,563 to 4,044,056
_3462
Pf6N2E2_3463 - Omega-amino acid--pyruvate aminotransferase (EC 2.6.1.18), at 4,044,128 to 4,045,498
_3463
Position (kb)
4042
4043
4044
4045 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4042.447 kb on + strand at 4042.557 kb on + strand at 4042.557 kb on + strand at 4042.557 kb on + strand at 4042.558 kb on - strand at 4042.564 kb on + strand at 4042.565 kb on - strand at 4042.645 kb on + strand at 4042.646 kb on - strand at 4042.646 kb on - strand at 4042.646 kb on - strand at 4042.720 kb on + strand, within Pf6N2E2_3462 at 4042.720 kb on + strand, within Pf6N2E2_3462 at 4042.721 kb on - strand, within Pf6N2E2_3462 at 4042.919 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.921 kb on + strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4042.922 kb on - strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.049 kb on + strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.050 kb on - strand, within Pf6N2E2_3462 at 4043.051 kb on + strand, within Pf6N2E2_3462 at 4043.051 kb on + strand, within Pf6N2E2_3462 at 4043.051 kb on + strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.052 kb on - strand, within Pf6N2E2_3462 at 4043.053 kb on + strand, within Pf6N2E2_3462 at 4043.053 kb on + strand, within Pf6N2E2_3462 at 4043.053 kb on + strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.054 kb on - strand, within Pf6N2E2_3462 at 4043.240 kb on - strand, within Pf6N2E2_3462 at 4043.359 kb on + strand, within Pf6N2E2_3462 at 4043.359 kb on + strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.360 kb on - strand, within Pf6N2E2_3462 at 4043.365 kb on + strand, within Pf6N2E2_3462 at 4043.366 kb on - strand, within Pf6N2E2_3462 at 4043.366 kb on - strand, within Pf6N2E2_3462 at 4043.581 kb on + strand, within Pf6N2E2_3462 at 4043.582 kb on - strand, within Pf6N2E2_3462 at 4043.582 kb on - strand, within Pf6N2E2_3462 at 4043.582 kb on - strand, within Pf6N2E2_3462 at 4043.679 kb on + strand, within Pf6N2E2_3462 at 4043.679 kb on + strand, within Pf6N2E2_3462 at 4043.679 kb on + strand, within Pf6N2E2_3462 at 4043.680 kb on - strand, within Pf6N2E2_3462 at 4043.692 kb on + strand, within Pf6N2E2_3462 at 4043.813 kb on + strand, within Pf6N2E2_3462 at 4043.814 kb on - strand, within Pf6N2E2_3462 at 4043.882 kb on + strand, within Pf6N2E2_3462 at 4043.882 kb on + strand, within Pf6N2E2_3462 at 4043.882 kb on + strand, within Pf6N2E2_3462 at 4043.883 kb on - strand, within Pf6N2E2_3462 at 4043.883 kb on - strand, within Pf6N2E2_3462 at 4043.883 kb on - strand, within Pf6N2E2_3462 at 4043.961 kb on - strand at 4043.961 kb on - strand at 4044.072 kb on - strand at 4044.102 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.112 kb on + strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.113 kb on - strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.240 kb on + strand at 4044.241 kb on - strand at 4044.241 kb on - strand at 4044.241 kb on - strand at 4044.337 kb on - strand, within Pf6N2E2_3463 at 4044.337 kb on - strand, within Pf6N2E2_3463 at 4044.337 kb on - strand, within Pf6N2E2_3463 at 4044.340 kb on + strand, within Pf6N2E2_3463 at 4044.340 kb on + strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.341 kb on - strand, within Pf6N2E2_3463 at 4044.492 kb on + strand, within Pf6N2E2_3463 at 4044.492 kb on + strand, within Pf6N2E2_3463 at 4044.492 kb on + strand, within Pf6N2E2_3463 at 4044.493 kb on - strand, within Pf6N2E2_3463 at 4044.499 kb on - strand, within Pf6N2E2_3463 at 4044.555 kb on + strand, within Pf6N2E2_3463 at 4044.555 kb on + strand, within Pf6N2E2_3463 at 4044.555 kb on + strand, within Pf6N2E2_3463 at 4044.556 kb on - strand, within Pf6N2E2_3463 at 4044.754 kb on - strand, within Pf6N2E2_3463 at 4044.754 kb on - strand, within Pf6N2E2_3463 at 4044.754 kb on - strand, within Pf6N2E2_3463 at 4044.900 kb on + strand, within Pf6N2E2_3463 at 4044.900 kb on + strand, within Pf6N2E2_3463 at 4044.900 kb on + strand, within Pf6N2E2_3463 at 4045.021 kb on - strand, within Pf6N2E2_3463 at 4045.021 kb on - strand, within Pf6N2E2_3463
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,042,447 + +0.9 4,042,557 + -0.0 4,042,557 + -0.3 4,042,557 + +0.5 4,042,558 - -0.0 4,042,564 + +0.1 4,042,565 - -2.1 4,042,645 + -0.9 4,042,646 - -1.2 4,042,646 - -1.7 4,042,646 - -3.4 4,042,720 + Pf6N2E2_3462 0.11 +0.4 4,042,720 + Pf6N2E2_3462 0.11 -2.5 4,042,721 - Pf6N2E2_3462 0.11 -0.6 4,042,919 + Pf6N2E2_3462 0.24 +0.0 4,042,921 + Pf6N2E2_3462 0.24 -0.3 4,042,921 + Pf6N2E2_3462 0.24 -1.3 4,042,921 + Pf6N2E2_3462 0.24 +0.6 4,042,921 + Pf6N2E2_3462 0.24 -1.4 4,042,921 + Pf6N2E2_3462 0.24 -0.9 4,042,921 + Pf6N2E2_3462 0.24 +0.7 4,042,921 + Pf6N2E2_3462 0.24 -0.3 4,042,921 + Pf6N2E2_3462 0.24 -0.8 4,042,921 + Pf6N2E2_3462 0.24 -0.1 4,042,922 - Pf6N2E2_3462 0.24 +0.3 4,042,922 - Pf6N2E2_3462 0.24 -2.1 4,042,922 - Pf6N2E2_3462 0.24 +0.2 4,042,922 - Pf6N2E2_3462 0.24 -0.2 4,042,922 - Pf6N2E2_3462 0.24 +0.2 4,042,922 - Pf6N2E2_3462 0.24 +0.6 4,042,922 - Pf6N2E2_3462 0.24 -1.8 4,042,922 - Pf6N2E2_3462 0.24 -0.2 4,042,922 - Pf6N2E2_3462 0.24 -0.2 4,043,049 + Pf6N2E2_3462 0.33 +0.4 4,043,049 + Pf6N2E2_3462 0.33 +1.1 4,043,049 + Pf6N2E2_3462 0.33 -1.6 4,043,049 + Pf6N2E2_3462 0.33 -0.9 4,043,049 + Pf6N2E2_3462 0.33 +1.0 4,043,049 + Pf6N2E2_3462 0.33 +0.5 4,043,049 + Pf6N2E2_3462 0.33 +0.1 4,043,049 + Pf6N2E2_3462 0.33 -0.1 4,043,049 + Pf6N2E2_3462 0.33 -0.0 4,043,049 + Pf6N2E2_3462 0.33 +0.3 4,043,049 + Pf6N2E2_3462 0.33 -1.1 4,043,049 + Pf6N2E2_3462 0.33 +0.8 4,043,049 + Pf6N2E2_3462 0.33 -2.2 4,043,050 - Pf6N2E2_3462 0.33 -0.1 4,043,050 - Pf6N2E2_3462 0.33 -0.2 4,043,050 - Pf6N2E2_3462 0.33 -1.6 4,043,050 - Pf6N2E2_3462 0.33 -0.7 4,043,050 - Pf6N2E2_3462 0.33 -1.0 4,043,050 - Pf6N2E2_3462 0.33 -0.5 4,043,050 - Pf6N2E2_3462 0.33 +1.1 4,043,050 - Pf6N2E2_3462 0.33 -0.3 4,043,050 - Pf6N2E2_3462 0.33 -1.4 4,043,050 - Pf6N2E2_3462 0.33 +0.7 4,043,050 - Pf6N2E2_3462 0.33 -0.1 4,043,050 - Pf6N2E2_3462 0.33 -0.2 4,043,050 - Pf6N2E2_3462 0.33 -0.6 4,043,051 + Pf6N2E2_3462 0.33 +0.5 4,043,051 + Pf6N2E2_3462 0.33 -0.1 4,043,051 + Pf6N2E2_3462 0.33 -0.8 4,043,052 - Pf6N2E2_3462 0.33 -0.4 4,043,052 - Pf6N2E2_3462 0.33 -0.3 4,043,052 - Pf6N2E2_3462 0.33 -2.3 4,043,052 - Pf6N2E2_3462 0.33 -1.1 4,043,052 - Pf6N2E2_3462 0.33 +0.4 4,043,052 - Pf6N2E2_3462 0.33 -0.5 4,043,052 - Pf6N2E2_3462 0.33 -0.6 4,043,052 - Pf6N2E2_3462 0.33 +0.2 4,043,052 - Pf6N2E2_3462 0.33 -0.2 4,043,052 - Pf6N2E2_3462 0.33 -0.3 4,043,052 - Pf6N2E2_3462 0.33 -0.3 4,043,053 + Pf6N2E2_3462 0.33 -0.5 4,043,053 + Pf6N2E2_3462 0.33 -0.0 4,043,053 + Pf6N2E2_3462 0.33 -1.0 4,043,054 - Pf6N2E2_3462 0.33 -0.0 4,043,054 - Pf6N2E2_3462 0.33 -1.1 4,043,054 - Pf6N2E2_3462 0.33 +0.2 4,043,054 - Pf6N2E2_3462 0.33 -1.7 4,043,240 - Pf6N2E2_3462 0.45 -1.8 4,043,359 + Pf6N2E2_3462 0.53 -0.2 4,043,359 + Pf6N2E2_3462 0.53 -0.6 4,043,360 - Pf6N2E2_3462 0.53 -0.7 4,043,360 - Pf6N2E2_3462 0.53 -0.8 4,043,360 - Pf6N2E2_3462 0.53 -1.4 4,043,360 - Pf6N2E2_3462 0.53 -1.8 4,043,360 - Pf6N2E2_3462 0.53 -0.9 4,043,360 - Pf6N2E2_3462 0.53 -1.0 4,043,360 - Pf6N2E2_3462 0.53 -0.0 4,043,360 - Pf6N2E2_3462 0.53 +0.3 4,043,365 + Pf6N2E2_3462 0.54 +1.5 4,043,366 - Pf6N2E2_3462 0.54 -0.7 4,043,366 - Pf6N2E2_3462 0.54 -0.8 4,043,581 + Pf6N2E2_3462 0.68 +1.4 4,043,582 - Pf6N2E2_3462 0.68 -1.3 4,043,582 - Pf6N2E2_3462 0.68 -0.1 4,043,582 - Pf6N2E2_3462 0.68 +0.1 4,043,679 + Pf6N2E2_3462 0.75 +0.3 4,043,679 + Pf6N2E2_3462 0.75 +1.1 4,043,679 + Pf6N2E2_3462 0.75 -2.4 4,043,680 - Pf6N2E2_3462 0.75 -1.8 4,043,692 + Pf6N2E2_3462 0.76 +2.2 4,043,813 + Pf6N2E2_3462 0.84 +0.4 4,043,814 - Pf6N2E2_3462 0.84 -0.8 4,043,882 + Pf6N2E2_3462 0.88 -0.6 4,043,882 + Pf6N2E2_3462 0.88 -1.6 4,043,882 + Pf6N2E2_3462 0.88 -0.2 4,043,883 - Pf6N2E2_3462 0.88 -0.7 4,043,883 - Pf6N2E2_3462 0.88 -0.9 4,043,883 - Pf6N2E2_3462 0.88 -0.0 4,043,961 - -1.4 4,043,961 - -2.2 4,044,072 - +0.4 4,044,102 + -0.9 4,044,112 + -0.6 4,044,112 + -0.5 4,044,112 + -0.2 4,044,112 + +1.4 4,044,112 + -0.7 4,044,112 + +0.4 4,044,112 + -1.2 4,044,112 + -0.1 4,044,112 + +0.4 4,044,113 - +1.2 4,044,113 - -1.0 4,044,113 - -0.1 4,044,113 - -0.3 4,044,113 - -1.5 4,044,113 - +0.0 4,044,113 - -0.4 4,044,240 + +0.1 4,044,240 + +0.5 4,044,240 + +0.1 4,044,240 + +0.0 4,044,240 + +0.4 4,044,240 + +0.7 4,044,240 + +0.8 4,044,240 + +1.3 4,044,240 + +0.8 4,044,241 - -1.4 4,044,241 - -0.4 4,044,241 - +0.9 4,044,337 - Pf6N2E2_3463 0.15 +0.1 4,044,337 - Pf6N2E2_3463 0.15 +1.5 4,044,337 - Pf6N2E2_3463 0.15 +0.5 4,044,340 + Pf6N2E2_3463 0.15 +0.2 4,044,340 + Pf6N2E2_3463 0.15 +0.4 4,044,341 - Pf6N2E2_3463 0.16 +1.0 4,044,341 - Pf6N2E2_3463 0.16 -0.5 4,044,341 - Pf6N2E2_3463 0.16 -0.9 4,044,341 - Pf6N2E2_3463 0.16 -2.4 4,044,492 + Pf6N2E2_3463 0.27 +0.8 4,044,492 + Pf6N2E2_3463 0.27 +1.6 4,044,492 + Pf6N2E2_3463 0.27 -0.1 4,044,493 - Pf6N2E2_3463 0.27 +0.3 4,044,499 - Pf6N2E2_3463 0.27 -0.5 4,044,555 + Pf6N2E2_3463 0.31 -0.2 4,044,555 + Pf6N2E2_3463 0.31 +0.3 4,044,555 + Pf6N2E2_3463 0.31 -0.8 4,044,556 - Pf6N2E2_3463 0.31 +0.4 4,044,754 - Pf6N2E2_3463 0.46 +0.5 4,044,754 - Pf6N2E2_3463 0.46 +0.3 4,044,754 - Pf6N2E2_3463 0.46 +0.5 4,044,900 + Pf6N2E2_3463 0.56 +0.4 4,044,900 + Pf6N2E2_3463 0.56 +0.5 4,044,900 + Pf6N2E2_3463 0.56 -0.0 4,045,021 - Pf6N2E2_3463 0.65 -0.2 4,045,021 - Pf6N2E2_3463 0.65 +0.8
Or see this region's nucleotide sequence