Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_216

Experiment: L-Asparagine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_215 and Pf6N2E2_216 are separated by 225 nucleotidesPf6N2E2_216 and Pf6N2E2_217 overlap by 4 nucleotides Pf6N2E2_215 - Outer membrane protein assembly factor YaeT precursor, at 271,909 to 274,275 _215 Pf6N2E2_216 - Copper-sensing two-component system response regulator CpxR, at 274,501 to 275,184 _216 Pf6N2E2_217 - Signal transduction histidine kinase, at 275,181 to 276,443 _217 Position (kb) 274 275 276Strain fitness (log2 ratio) -2 -1 0 1at 274.310 kb on + strandat 274.310 kb on + strandat 274.310 kb on + strandat 274.310 kb on + strandat 274.310 kb on + strandat 274.311 kb on - strandat 274.311 kb on - strandat 274.311 kb on - strandat 274.311 kb on - strandat 274.380 kb on + strandat 274.422 kb on + strandat 274.423 kb on - strandat 274.426 kb on + strandat 274.485 kb on + strandat 274.485 kb on + strandat 274.511 kb on + strandat 274.511 kb on + strandat 274.512 kb on - strandat 274.512 kb on - strandat 274.638 kb on + strand, within Pf6N2E2_216at 274.638 kb on + strand, within Pf6N2E2_216at 274.638 kb on + strand, within Pf6N2E2_216at 274.639 kb on - strand, within Pf6N2E2_216at 274.640 kb on - strand, within Pf6N2E2_216at 274.792 kb on + strand, within Pf6N2E2_216at 274.792 kb on + strand, within Pf6N2E2_216at 274.792 kb on + strand, within Pf6N2E2_216at 274.793 kb on - strand, within Pf6N2E2_216at 275.066 kb on - strand, within Pf6N2E2_216at 275.164 kb on + strandat 275.322 kb on - strand, within Pf6N2E2_217at 275.335 kb on + strand, within Pf6N2E2_217at 275.335 kb on + strand, within Pf6N2E2_217at 275.336 kb on - strand, within Pf6N2E2_217at 275.336 kb on - strand, within Pf6N2E2_217at 275.415 kb on + strand, within Pf6N2E2_217at 275.467 kb on - strand, within Pf6N2E2_217at 275.607 kb on + strand, within Pf6N2E2_217at 275.607 kb on + strand, within Pf6N2E2_217at 275.620 kb on - strand, within Pf6N2E2_217at 275.779 kb on + strand, within Pf6N2E2_217

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Asparagine (N)
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274,310 + +0.2
274,310 + +0.1
274,310 + -0.8
274,310 + +0.9
274,310 + +0.2
274,311 - -1.4
274,311 - +0.5
274,311 - +0.2
274,311 - -0.0
274,380 + -0.1
274,422 + -0.2
274,423 - -0.3
274,426 + +0.2
274,485 + +0.3
274,485 + +0.5
274,511 + +0.2
274,511 + -0.4
274,512 - -0.3
274,512 - +0.4
274,638 + Pf6N2E2_216 0.20 -1.7
274,638 + Pf6N2E2_216 0.20 -1.4
274,638 + Pf6N2E2_216 0.20 -0.9
274,639 - Pf6N2E2_216 0.20 -1.4
274,640 - Pf6N2E2_216 0.20 -0.6
274,792 + Pf6N2E2_216 0.43 -0.5
274,792 + Pf6N2E2_216 0.43 -1.8
274,792 + Pf6N2E2_216 0.43 -1.2
274,793 - Pf6N2E2_216 0.43 -0.6
275,066 - Pf6N2E2_216 0.83 +1.4
275,164 + +0.2
275,322 - Pf6N2E2_217 0.11 -1.5
275,335 + Pf6N2E2_217 0.12 +0.2
275,335 + Pf6N2E2_217 0.12 +0.5
275,336 - Pf6N2E2_217 0.12 -1.1
275,336 - Pf6N2E2_217 0.12 -1.4
275,415 + Pf6N2E2_217 0.19 +0.1
275,467 - Pf6N2E2_217 0.23 +0.4
275,607 + Pf6N2E2_217 0.34 +0.2
275,607 + Pf6N2E2_217 0.34 -0.0
275,620 - Pf6N2E2_217 0.35 +0.7
275,779 + Pf6N2E2_217 0.47 -0.0

Or see this region's nucleotide sequence