Experiment: L-Aspartic Acid (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1205 and Pf6N2E2_1206 are separated by 242 nucleotides Pf6N2E2_1206 and Pf6N2E2_1207 are separated by 212 nucleotides
Pf6N2E2_1205 - Transcriptional regulator, AraC family, at 1,411,599 to 1,412,507
_1205
Pf6N2E2_1206 - protein of unknown function UPF0153, at 1,412,750 to 1,413,511
_1206
Pf6N2E2_1207 - Epoxide hydrolase (EC 3.3.2.9), at 1,413,724 to 1,414,797
_1207
Position (kb)
1412
1413
1414 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1411.753 kb on - strand, within Pf6N2E2_1205 at 1411.753 kb on - strand, within Pf6N2E2_1205 at 1411.771 kb on + strand, within Pf6N2E2_1205 at 1411.860 kb on - strand, within Pf6N2E2_1205 at 1411.860 kb on - strand, within Pf6N2E2_1205 at 1412.091 kb on + strand, within Pf6N2E2_1205 at 1412.091 kb on + strand, within Pf6N2E2_1205 at 1412.279 kb on - strand, within Pf6N2E2_1205 at 1412.286 kb on + strand, within Pf6N2E2_1205 at 1412.347 kb on + strand, within Pf6N2E2_1205 at 1412.347 kb on + strand, within Pf6N2E2_1205 at 1412.348 kb on - strand, within Pf6N2E2_1205 at 1412.348 kb on - strand, within Pf6N2E2_1205 at 1412.574 kb on - strand at 1412.702 kb on + strand at 1412.703 kb on - strand at 1412.703 kb on - strand at 1412.703 kb on - strand at 1412.703 kb on - strand at 1412.703 kb on - strand at 1412.769 kb on + strand at 1412.931 kb on + strand, within Pf6N2E2_1206 at 1412.931 kb on + strand, within Pf6N2E2_1206 at 1412.931 kb on + strand, within Pf6N2E2_1206 at 1412.931 kb on + strand, within Pf6N2E2_1206 at 1412.932 kb on - strand, within Pf6N2E2_1206 at 1412.932 kb on - strand, within Pf6N2E2_1206 at 1413.166 kb on - strand, within Pf6N2E2_1206 at 1413.195 kb on + strand, within Pf6N2E2_1206 at 1413.195 kb on + strand at 1413.195 kb on + strand, within Pf6N2E2_1206 at 1413.195 kb on + strand, within Pf6N2E2_1206 at 1413.196 kb on - strand, within Pf6N2E2_1206 at 1413.196 kb on - strand, within Pf6N2E2_1206 at 1413.332 kb on - strand, within Pf6N2E2_1206 at 1413.372 kb on - strand, within Pf6N2E2_1206 at 1413.399 kb on - strand, within Pf6N2E2_1206 at 1413.496 kb on + strand at 1413.529 kb on + strand at 1413.585 kb on + strand at 1413.585 kb on + strand at 1413.585 kb on + strand at 1413.585 kb on + strand at 1413.585 kb on + strand at 1413.585 kb on + strand at 1413.586 kb on - strand at 1413.586 kb on - strand at 1413.656 kb on + strand at 1413.657 kb on - strand at 1413.824 kb on + strand at 1413.953 kb on + strand, within Pf6N2E2_1207 at 1413.954 kb on - strand, within Pf6N2E2_1207 at 1413.992 kb on + strand, within Pf6N2E2_1207 at 1414.169 kb on + strand, within Pf6N2E2_1207
Per-strain Table
Position Strand Gene LocusTag Fraction L-Aspartic Acid (N) remove 1,411,753 - Pf6N2E2_1205 0.17 -0.9 1,411,753 - Pf6N2E2_1205 0.17 -0.4 1,411,771 + Pf6N2E2_1205 0.19 +1.9 1,411,860 - Pf6N2E2_1205 0.29 -0.2 1,411,860 - Pf6N2E2_1205 0.29 -0.4 1,412,091 + Pf6N2E2_1205 0.54 +0.0 1,412,091 + Pf6N2E2_1205 0.54 -0.4 1,412,279 - Pf6N2E2_1205 0.75 -1.1 1,412,286 + Pf6N2E2_1205 0.76 +0.3 1,412,347 + Pf6N2E2_1205 0.82 +0.8 1,412,347 + Pf6N2E2_1205 0.82 -0.5 1,412,348 - Pf6N2E2_1205 0.82 +0.0 1,412,348 - Pf6N2E2_1205 0.82 -0.8 1,412,574 - +0.4 1,412,702 + +2.8 1,412,703 - +0.1 1,412,703 - +0.4 1,412,703 - +0.8 1,412,703 - +0.6 1,412,703 - +0.8 1,412,769 + +0.6 1,412,931 + Pf6N2E2_1206 0.24 +0.2 1,412,931 + Pf6N2E2_1206 0.24 -0.8 1,412,931 + Pf6N2E2_1206 0.24 -0.3 1,412,931 + Pf6N2E2_1206 0.24 +0.2 1,412,932 - Pf6N2E2_1206 0.24 -1.5 1,412,932 - Pf6N2E2_1206 0.24 +0.8 1,413,166 - Pf6N2E2_1206 0.55 +0.3 1,413,195 + Pf6N2E2_1206 0.58 +1.6 1,413,195 + +1.2 1,413,195 + Pf6N2E2_1206 0.58 +0.4 1,413,195 + Pf6N2E2_1206 0.58 +1.4 1,413,196 - Pf6N2E2_1206 0.59 +0.3 1,413,196 - Pf6N2E2_1206 0.59 +1.2 1,413,332 - Pf6N2E2_1206 0.76 +0.8 1,413,372 - Pf6N2E2_1206 0.82 +0.1 1,413,399 - Pf6N2E2_1206 0.85 +1.6 1,413,496 + +0.8 1,413,529 + +0.4 1,413,585 + -0.5 1,413,585 + +0.4 1,413,585 + +0.3 1,413,585 + -0.4 1,413,585 + -0.5 1,413,585 + -1.1 1,413,586 - -0.3 1,413,586 - -0.2 1,413,656 + -0.5 1,413,657 - -0.6 1,413,824 + -2.6 1,413,953 + Pf6N2E2_1207 0.21 -0.5 1,413,954 - Pf6N2E2_1207 0.21 +1.8 1,413,992 + Pf6N2E2_1207 0.25 -0.7 1,414,169 + Pf6N2E2_1207 0.41 -1.3
Or see this region's nucleotide sequence