Experiment: L-Histidine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4318 and Pf6N2E2_4319 are separated by 248 nucleotides Pf6N2E2_4319 and Pf6N2E2_4320 are separated by 35 nucleotides
Pf6N2E2_4318 - VgrG-3 protein, at 5,038,983 to 5,040,914
_4318
Pf6N2E2_4319 - FIG00960169: hypothetical protein, at 5,041,163 to 5,041,597
_4319
Pf6N2E2_4320 - Rhs family protein, at 5,041,633 to 5,042,898
_4320
Position (kb)
5041
5042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5040.314 kb on - strand, within Pf6N2E2_4318 at 5040.316 kb on + strand, within Pf6N2E2_4318 at 5040.410 kb on - strand, within Pf6N2E2_4318 at 5040.410 kb on - strand, within Pf6N2E2_4318 at 5040.410 kb on - strand, within Pf6N2E2_4318 at 5040.413 kb on - strand, within Pf6N2E2_4318 at 5040.413 kb on - strand, within Pf6N2E2_4318 at 5040.413 kb on - strand, within Pf6N2E2_4318 at 5040.478 kb on + strand, within Pf6N2E2_4318 at 5040.478 kb on + strand, within Pf6N2E2_4318 at 5040.803 kb on - strand at 5040.803 kb on - strand at 5040.909 kb on + strand at 5040.909 kb on + strand at 5040.909 kb on + strand at 5040.910 kb on - strand at 5040.925 kb on + strand at 5041.035 kb on + strand at 5041.035 kb on + strand at 5041.035 kb on + strand at 5041.036 kb on - strand at 5041.036 kb on - strand at 5041.057 kb on + strand at 5041.057 kb on + strand at 5041.057 kb on + strand at 5041.057 kb on + strand at 5041.057 kb on + strand at 5041.057 kb on + strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.058 kb on - strand at 5041.108 kb on + strand at 5041.108 kb on + strand at 5041.109 kb on - strand at 5041.116 kb on + strand at 5041.117 kb on - strand at 5041.159 kb on + strand at 5041.231 kb on + strand, within Pf6N2E2_4319 at 5041.232 kb on - strand, within Pf6N2E2_4319 at 5041.261 kb on + strand, within Pf6N2E2_4319 at 5041.262 kb on - strand, within Pf6N2E2_4319 at 5041.322 kb on + strand, within Pf6N2E2_4319 at 5041.414 kb on + strand, within Pf6N2E2_4319 at 5041.414 kb on + strand, within Pf6N2E2_4319 at 5041.414 kb on + strand, within Pf6N2E2_4319 at 5041.414 kb on + strand, within Pf6N2E2_4319 at 5041.428 kb on - strand, within Pf6N2E2_4319 at 5041.428 kb on - strand, within Pf6N2E2_4319 at 5041.483 kb on + strand, within Pf6N2E2_4319 at 5041.483 kb on + strand, within Pf6N2E2_4319 at 5041.605 kb on + strand at 5041.606 kb on - strand at 5041.606 kb on - strand at 5041.606 kb on - strand at 5041.619 kb on + strand at 5041.619 kb on + strand at 5041.619 kb on + strand at 5041.620 kb on - strand at 5041.625 kb on + strand at 5041.625 kb on + strand at 5041.625 kb on + strand at 5041.625 kb on + strand at 5041.625 kb on + strand at 5041.625 kb on + strand at 5041.625 kb on + strand at 5041.625 kb on + strand at 5041.626 kb on - strand at 5041.626 kb on - strand at 5041.626 kb on - strand at 5041.626 kb on - strand at 5041.626 kb on - strand at 5041.626 kb on - strand at 5041.626 kb on - strand at 5041.626 kb on - strand at 5041.670 kb on + strand at 5041.671 kb on - strand at 5042.283 kb on + strand, within Pf6N2E2_4320 at 5042.284 kb on - strand, within Pf6N2E2_4320 at 5042.303 kb on + strand, within Pf6N2E2_4320 at 5042.425 kb on + strand, within Pf6N2E2_4320 at 5042.425 kb on + strand, within Pf6N2E2_4320 at 5042.426 kb on - strand, within Pf6N2E2_4320 at 5042.494 kb on + strand, within Pf6N2E2_4320 at 5042.494 kb on + strand, within Pf6N2E2_4320 at 5042.494 kb on + strand, within Pf6N2E2_4320 at 5042.494 kb on + strand, within Pf6N2E2_4320 at 5042.494 kb on + strand, within Pf6N2E2_4320 at 5042.494 kb on + strand, within Pf6N2E2_4320 at 5042.495 kb on - strand, within Pf6N2E2_4320 at 5042.495 kb on - strand, within Pf6N2E2_4320 at 5042.495 kb on - strand, within Pf6N2E2_4320 at 5042.495 kb on - strand, within Pf6N2E2_4320 at 5042.495 kb on - strand, within Pf6N2E2_4320 at 5042.495 kb on - strand, within Pf6N2E2_4320 at 5042.509 kb on + strand, within Pf6N2E2_4320 at 5042.509 kb on + strand, within Pf6N2E2_4320 at 5042.509 kb on + strand, within Pf6N2E2_4320 at 5042.509 kb on + strand, within Pf6N2E2_4320 at 5042.510 kb on - strand, within Pf6N2E2_4320 at 5042.510 kb on - strand, within Pf6N2E2_4320 at 5042.510 kb on - strand, within Pf6N2E2_4320 at 5042.510 kb on - strand, within Pf6N2E2_4320 at 5042.582 kb on - strand, within Pf6N2E2_4320 at 5042.582 kb on - strand, within Pf6N2E2_4320
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (N) remove 5,040,314 - Pf6N2E2_4318 0.69 +0.5 5,040,316 + Pf6N2E2_4318 0.69 +0.0 5,040,410 - Pf6N2E2_4318 0.74 -0.3 5,040,410 - Pf6N2E2_4318 0.74 -0.5 5,040,410 - Pf6N2E2_4318 0.74 -2.0 5,040,413 - Pf6N2E2_4318 0.74 -0.1 5,040,413 - Pf6N2E2_4318 0.74 +2.6 5,040,413 - Pf6N2E2_4318 0.74 +0.3 5,040,478 + Pf6N2E2_4318 0.77 +1.0 5,040,478 + Pf6N2E2_4318 0.77 +0.2 5,040,803 - +0.1 5,040,803 - -0.5 5,040,909 + -2.6 5,040,909 + +0.2 5,040,909 + +0.7 5,040,910 - +0.5 5,040,925 + -0.2 5,041,035 + +0.1 5,041,035 + -1.4 5,041,035 + -0.1 5,041,036 - +1.0 5,041,036 - +0.3 5,041,057 + +0.1 5,041,057 + +1.7 5,041,057 + +0.2 5,041,057 + +0.2 5,041,057 + +0.8 5,041,057 + -1.9 5,041,058 - +0.2 5,041,058 - -0.3 5,041,058 - -1.2 5,041,058 - +0.3 5,041,058 - -0.2 5,041,058 - -0.4 5,041,058 - -0.3 5,041,058 - -0.2 5,041,058 - -0.3 5,041,108 + -0.2 5,041,108 + +0.2 5,041,109 - +0.0 5,041,116 + -0.9 5,041,117 - +0.3 5,041,159 + -0.9 5,041,231 + Pf6N2E2_4319 0.16 -0.2 5,041,232 - Pf6N2E2_4319 0.16 -0.9 5,041,261 + Pf6N2E2_4319 0.23 -0.3 5,041,262 - Pf6N2E2_4319 0.23 -1.5 5,041,322 + Pf6N2E2_4319 0.37 -0.2 5,041,414 + Pf6N2E2_4319 0.58 +0.5 5,041,414 + Pf6N2E2_4319 0.58 -0.9 5,041,414 + Pf6N2E2_4319 0.58 -0.1 5,041,414 + Pf6N2E2_4319 0.58 -0.6 5,041,428 - Pf6N2E2_4319 0.61 +0.0 5,041,428 - Pf6N2E2_4319 0.61 +1.0 5,041,483 + Pf6N2E2_4319 0.74 +0.3 5,041,483 + Pf6N2E2_4319 0.74 +0.3 5,041,605 + +0.3 5,041,606 - +0.6 5,041,606 - -0.0 5,041,606 - +0.6 5,041,619 + -0.8 5,041,619 + -2.8 5,041,619 + -0.5 5,041,620 - +0.7 5,041,625 + +0.7 5,041,625 + -0.4 5,041,625 + -0.2 5,041,625 + -1.0 5,041,625 + +0.3 5,041,625 + -0.8 5,041,625 + -0.1 5,041,625 + +0.7 5,041,626 - -0.2 5,041,626 - -0.5 5,041,626 - -0.8 5,041,626 - -0.6 5,041,626 - -0.4 5,041,626 - +0.8 5,041,626 - -0.5 5,041,626 - -0.5 5,041,670 + -0.3 5,041,671 - +0.3 5,042,283 + Pf6N2E2_4320 0.51 -0.0 5,042,284 - Pf6N2E2_4320 0.51 -1.9 5,042,303 + Pf6N2E2_4320 0.53 -0.4 5,042,425 + Pf6N2E2_4320 0.63 +0.6 5,042,425 + Pf6N2E2_4320 0.63 +0.4 5,042,426 - Pf6N2E2_4320 0.63 +0.6 5,042,494 + Pf6N2E2_4320 0.68 +0.5 5,042,494 + Pf6N2E2_4320 0.68 +0.9 5,042,494 + Pf6N2E2_4320 0.68 -0.3 5,042,494 + Pf6N2E2_4320 0.68 +0.6 5,042,494 + Pf6N2E2_4320 0.68 +0.3 5,042,494 + Pf6N2E2_4320 0.68 -0.1 5,042,495 - Pf6N2E2_4320 0.68 +0.2 5,042,495 - Pf6N2E2_4320 0.68 +0.7 5,042,495 - Pf6N2E2_4320 0.68 +0.3 5,042,495 - Pf6N2E2_4320 0.68 -0.8 5,042,495 - Pf6N2E2_4320 0.68 +0.4 5,042,495 - Pf6N2E2_4320 0.68 +0.7 5,042,509 + Pf6N2E2_4320 0.69 +0.8 5,042,509 + Pf6N2E2_4320 0.69 -0.8 5,042,509 + Pf6N2E2_4320 0.69 -0.1 5,042,509 + Pf6N2E2_4320 0.69 +0.2 5,042,510 - Pf6N2E2_4320 0.69 +0.3 5,042,510 - Pf6N2E2_4320 0.69 -0.4 5,042,510 - Pf6N2E2_4320 0.69 -0.0 5,042,510 - Pf6N2E2_4320 0.69 -0.7 5,042,582 - Pf6N2E2_4320 0.75 -0.2 5,042,582 - Pf6N2E2_4320 0.75 +0.9
Or see this region's nucleotide sequence