Experiment: DEANONOate 0.000625 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_686 and Pf6N2E2_687 are separated by 253 nucleotides Pf6N2E2_687 and Pf6N2E2_688 are separated by 126 nucleotides Pf6N2E2_688 and Pf6N2E2_689 are separated by 198 nucleotides Pf6N2E2_689 and Pf6N2E2_690 are separated by 259 nucleotides
Pf6N2E2_686 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 792,843 to 793,577
_686
Pf6N2E2_687 - 4-oxalocrotonate tautomerase (EC 5.3.2.-), at 793,831 to 794,037
_687
Pf6N2E2_688 - Transcriptional regulator, LysR family, at 794,164 to 795,087
_688
Pf6N2E2_689 - FIG00956189: hypothetical protein, at 795,286 to 795,780
_689
Pf6N2E2_690 - Transcriptional regulator, LysR family, at 796,040 to 796,936
_690
Position (kb) 794
795
796 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 793.229 kb on - strand, within Pf6N2E2_686 at 793.257 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.490 kb on + strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.491 kb on - strand, within Pf6N2E2_686 at 793.492 kb on + strand, within Pf6N2E2_686 at 793.679 kb on + strand at 793.691 kb on + strand at 793.691 kb on + strand at 793.691 kb on + strand at 793.833 kb on - strand at 793.888 kb on - strand, within Pf6N2E2_687 at 793.888 kb on - strand, within Pf6N2E2_687 at 793.888 kb on - strand, within Pf6N2E2_687 at 794.013 kb on + strand, within Pf6N2E2_687 at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.097 kb on + strand at 794.098 kb on - strand at 794.098 kb on - strand at 794.098 kb on - strand at 794.101 kb on + strand at 794.101 kb on + strand at 794.102 kb on - strand at 794.102 kb on - strand at 794.156 kb on - strand at 794.156 kb on - strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.201 kb on + strand at 794.202 kb on - strand at 794.202 kb on - strand at 794.202 kb on - strand at 794.374 kb on + strand, within Pf6N2E2_688 at 794.374 kb on + strand, within Pf6N2E2_688 at 794.375 kb on - strand, within Pf6N2E2_688 at 794.375 kb on - strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.485 kb on + strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.486 kb on - strand, within Pf6N2E2_688 at 794.692 kb on + strand, within Pf6N2E2_688 at 794.693 kb on - strand, within Pf6N2E2_688 at 794.822 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.839 kb on + strand, within Pf6N2E2_688 at 794.840 kb on - strand at 794.840 kb on - strand, within Pf6N2E2_688 at 794.840 kb on - strand, within Pf6N2E2_688 at 794.840 kb on - strand, within Pf6N2E2_688 at 794.908 kb on + strand, within Pf6N2E2_688 at 794.909 kb on - strand, within Pf6N2E2_688 at 794.951 kb on + strand, within Pf6N2E2_688 at 794.952 kb on - strand, within Pf6N2E2_688 at 795.001 kb on + strand at 795.017 kb on - strand at 795.017 kb on - strand at 795.116 kb on + strand at 795.216 kb on - strand at 795.427 kb on + strand, within Pf6N2E2_689 at 795.427 kb on + strand, within Pf6N2E2_689 at 795.548 kb on + strand, within Pf6N2E2_689 at 795.548 kb on + strand, within Pf6N2E2_689 at 795.740 kb on + strand at 795.740 kb on + strand at 795.740 kb on + strand at 795.741 kb on - strand at 795.741 kb on - strand at 795.741 kb on - strand at 795.755 kb on + strand at 795.755 kb on + strand at 795.755 kb on + strand at 795.756 kb on - strand at 795.756 kb on - strand at 795.756 kb on - strand at 795.756 kb on - strand at 795.820 kb on + strand at 795.821 kb on - strand at 795.821 kb on - strand at 796.023 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction DEANONOate 0.000625 M remove 793,229 - Pf6N2E2_686 0.53 -2.0 793,257 + Pf6N2E2_686 0.56 +1.8 793,490 + Pf6N2E2_686 0.88 +1.1 793,490 + Pf6N2E2_686 0.88 +0.4 793,490 + Pf6N2E2_686 0.88 +2.3 793,490 + Pf6N2E2_686 0.88 -0.0 793,491 - Pf6N2E2_686 0.88 -1.1 793,491 - Pf6N2E2_686 0.88 +0.5 793,491 - Pf6N2E2_686 0.88 +1.3 793,491 - Pf6N2E2_686 0.88 -1.2 793,491 - Pf6N2E2_686 0.88 -1.0 793,491 - Pf6N2E2_686 0.88 +0.8 793,491 - Pf6N2E2_686 0.88 +1.6 793,492 + Pf6N2E2_686 0.88 +1.0 793,679 + -0.6 793,691 + -1.5 793,691 + -0.2 793,691 + +0.6 793,833 - -0.0 793,888 - Pf6N2E2_687 0.28 -1.2 793,888 - Pf6N2E2_687 0.28 -0.2 793,888 - Pf6N2E2_687 0.28 -2.6 794,013 + Pf6N2E2_687 0.88 -0.5 794,097 + +3.3 794,097 + -1.8 794,097 + -0.5 794,097 + +0.7 794,097 + -2.3 794,097 + +0.4 794,097 + +0.9 794,098 - +0.8 794,098 - -2.5 794,098 - -2.9 794,101 + -0.5 794,101 + -3.1 794,102 - +0.7 794,102 - +0.6 794,156 - -1.8 794,156 - +0.5 794,201 + +0.3 794,201 + +0.9 794,201 + +1.0 794,201 + -3.5 794,201 + +1.1 794,201 + +0.0 794,202 - -1.3 794,202 - -0.2 794,202 - +1.1 794,374 + Pf6N2E2_688 0.23 -0.2 794,374 + Pf6N2E2_688 0.23 +1.5 794,375 - Pf6N2E2_688 0.23 -0.5 794,375 - Pf6N2E2_688 0.23 +1.2 794,485 + Pf6N2E2_688 0.35 +2.3 794,485 + Pf6N2E2_688 0.35 +0.2 794,485 + Pf6N2E2_688 0.35 +0.1 794,485 + Pf6N2E2_688 0.35 +1.3 794,486 - Pf6N2E2_688 0.35 +0.2 794,486 - Pf6N2E2_688 0.35 -1.4 794,486 - Pf6N2E2_688 0.35 +0.1 794,486 - Pf6N2E2_688 0.35 -1.4 794,692 + Pf6N2E2_688 0.57 +2.0 794,693 - Pf6N2E2_688 0.57 -1.2 794,822 + Pf6N2E2_688 0.71 +1.4 794,839 + Pf6N2E2_688 0.73 +1.5 794,839 + Pf6N2E2_688 0.73 -2.2 794,839 + Pf6N2E2_688 0.73 +1.1 794,839 + Pf6N2E2_688 0.73 +0.8 794,839 + Pf6N2E2_688 0.73 +0.3 794,840 - +2.7 794,840 - Pf6N2E2_688 0.73 -3.2 794,840 - Pf6N2E2_688 0.73 -0.1 794,840 - Pf6N2E2_688 0.73 +0.6 794,908 + Pf6N2E2_688 0.81 +0.9 794,909 - Pf6N2E2_688 0.81 +0.2 794,951 + Pf6N2E2_688 0.85 +1.5 794,952 - Pf6N2E2_688 0.85 +0.3 795,001 + -0.6 795,017 - +0.5 795,017 - -2.8 795,116 + -2.6 795,216 - +1.8 795,427 + Pf6N2E2_689 0.28 -2.9 795,427 + Pf6N2E2_689 0.28 -1.8 795,548 + Pf6N2E2_689 0.53 +0.5 795,548 + Pf6N2E2_689 0.53 +0.8 795,740 + -0.3 795,740 + +0.1 795,740 + +1.0 795,741 - -0.5 795,741 - +1.3 795,741 - -2.9 795,755 + +1.0 795,755 + +2.4 795,755 + -1.1 795,756 - -0.6 795,756 - +0.9 795,756 - +0.1 795,756 - +1.8 795,820 + +1.7 795,821 - -2.8 795,821 - +0.1 796,023 + +1.3
Or see this region's nucleotide sequence