Strain Fitness in Escherichia coli BL21 around ECD_00140

Experiment: EV116

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECD_00138 and ECD_00139 are separated by 34 nucleotidesECD_00139 and ECD_00140 are separated by 104 nucleotidesECD_00140 and ECD_00141 are separated by 369 nucleotidesECD_00141 and ECD_00142 overlap by 4 nucleotides ECD_00138: ECD_00138 - putative outer membrane usher protein, at 155,671 to 158,271 _00138 ECD_00139: ECD_00139 - putative periplasmic pilin chaperone, at 158,306 to 159,046 _00139 ECD_00140: ECD_00140 - putative fimbrial-like adhesin protein, at 159,151 to 159,735 _00140 ECD_00141: ECD_00141 - 2-amino-4-hydroxy-6-hydroxymethyldihyropteridine pyrophosphokinase, at 160,105 to 160,584 _00141 ECD_00142: ECD_00142 - poly(A) polymerase, at 160,581 to 161,945 _00142 Position (kb) 159 160Strain fitness (log2 ratio) -2 -1 0 1at 158.166 kb on - strandat 158.242 kb on - strandat 158.485 kb on + strand, within ECD_00139at 158.496 kb on + strand, within ECD_00139at 158.497 kb on - strand, within ECD_00139at 158.499 kb on + strand, within ECD_00139at 158.623 kb on + strand, within ECD_00139at 158.828 kb on - strand, within ECD_00139at 159.339 kb on + strand, within ECD_00140at 160.572 kb on - strandat 160.572 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction EV116
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158,166 - -0.0
158,242 - -0.6
158,485 + ECD_00139 0.24 +0.6
158,496 + ECD_00139 0.26 +1.1
158,497 - ECD_00139 0.26 -0.6
158,499 + ECD_00139 0.26 -0.3
158,623 + ECD_00139 0.43 -0.3
158,828 - ECD_00139 0.70 -1.9
159,339 + ECD_00140 0.32 -0.1
160,572 - +0.6
160,572 - +1.2

Or see this region's nucleotide sequence