Experiment: RCH2_defined_Glucose_noNitrogen in Eppendorf tube
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18360 and BWI76_RS18365 are separated by 95 nucleotides BWI76_RS18365 and BWI76_RS18370 are separated by 201 nucleotides
BWI76_RS18360: BWI76_RS18360 - aminocyclopropane-1-carboxylate deaminase/D-cysteine desulfhydrase family protein, at 3,811,260 to 3,812,246
_RS18360
BWI76_RS18365: BWI76_RS18365 - cystine ABC transporter substrate-binding protein, at 3,812,342 to 3,813,142
_RS18365
BWI76_RS18370: BWI76_RS18370 - alpha-amylase, at 3,813,344 to 3,814,831
_RS18370
Position (kb)
3812
3813
3814 Strain fitness (log2 ratio)
-1
0
1
2 at 3811.486 kb on + strand, within BWI76_RS18360 at 3811.800 kb on - strand, within BWI76_RS18360 at 3811.968 kb on - strand, within BWI76_RS18360 at 3812.259 kb on - strand at 3812.430 kb on + strand, within BWI76_RS18365 at 3812.451 kb on + strand, within BWI76_RS18365 at 3812.451 kb on + strand, within BWI76_RS18365 at 3812.459 kb on - strand, within BWI76_RS18365 at 3812.777 kb on - strand, within BWI76_RS18365 at 3812.997 kb on - strand, within BWI76_RS18365 at 3813.035 kb on - strand, within BWI76_RS18365 at 3813.130 kb on - strand at 3813.183 kb on - strand at 3813.184 kb on + strand at 3813.267 kb on - strand at 3813.327 kb on + strand at 3813.330 kb on - strand at 3813.379 kb on + strand at 3813.466 kb on + strand at 3813.507 kb on + strand, within BWI76_RS18370 at 3813.550 kb on + strand, within BWI76_RS18370 at 3813.642 kb on + strand, within BWI76_RS18370 at 3813.761 kb on + strand, within BWI76_RS18370 at 3813.768 kb on - strand, within BWI76_RS18370 at 3813.769 kb on - strand, within BWI76_RS18370 at 3813.828 kb on + strand, within BWI76_RS18370 at 3814.104 kb on - strand, within BWI76_RS18370
Per-strain Table
Position Strand Gene LocusTag Fraction RCH2_defined_Glucose_noNitrogen in Eppendorf tube remove 3,811,486 + BWI76_RS18360 0.23 -0.4 3,811,800 - BWI76_RS18360 0.55 -0.6 3,811,968 - BWI76_RS18360 0.72 -0.1 3,812,259 - -0.2 3,812,430 + BWI76_RS18365 0.11 -0.2 3,812,451 + BWI76_RS18365 0.14 -0.0 3,812,451 + BWI76_RS18365 0.14 +0.1 3,812,459 - BWI76_RS18365 0.15 +0.5 3,812,777 - BWI76_RS18365 0.54 +0.1 3,812,997 - BWI76_RS18365 0.82 -0.2 3,813,035 - BWI76_RS18365 0.87 +1.8 3,813,130 - +1.0 3,813,183 - -0.5 3,813,184 + +0.0 3,813,267 - +0.3 3,813,327 + +0.3 3,813,330 - +0.9 3,813,379 + +0.5 3,813,466 + -0.4 3,813,507 + BWI76_RS18370 0.11 -0.8 3,813,550 + BWI76_RS18370 0.14 -0.8 3,813,642 + BWI76_RS18370 0.20 +1.6 3,813,761 + BWI76_RS18370 0.28 +0.0 3,813,768 - BWI76_RS18370 0.28 +0.0 3,813,769 - BWI76_RS18370 0.29 +0.5 3,813,828 + BWI76_RS18370 0.33 +0.3 3,814,104 - BWI76_RS18370 0.51 -0.4
Or see this region's nucleotide sequence