Experiment: KB with Avocatin A 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1873 and Psyr_1874 are separated by 63 nucleotides Psyr_1874 and Psyr_1875 are separated by 123 nucleotides Psyr_1875 and Psyr_1876 are separated by 125 nucleotides
Psyr_1873: Psyr_1873 - Glutamine amidotransferase class-I, at 2,148,483 to 2,149,211
_1873
Psyr_1874: Psyr_1874 - Mg2+ transporter protein, CorA-like protein, at 2,149,275 to 2,150,246
_1874
Psyr_1875: Psyr_1875 - Enoyl-CoA hydratase/isomerase, at 2,150,370 to 2,151,059
_1875
Psyr_1876: Psyr_1876 - conserved hypothetical protein, at 2,151,185 to 2,151,478
_1876
Position (kb)
2149
2150
2151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2148.315 kb on + strand at 2148.373 kb on - strand at 2148.382 kb on - strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.482 kb on + strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.483 kb on - strand at 2148.490 kb on + strand at 2148.500 kb on + strand at 2148.552 kb on + strand at 2148.572 kb on - strand, within Psyr_1873 at 2148.572 kb on - strand, within Psyr_1873 at 2148.612 kb on + strand, within Psyr_1873 at 2148.613 kb on - strand, within Psyr_1873 at 2148.663 kb on + strand, within Psyr_1873 at 2148.664 kb on - strand, within Psyr_1873 at 2148.729 kb on + strand, within Psyr_1873 at 2148.729 kb on + strand, within Psyr_1873 at 2148.730 kb on - strand, within Psyr_1873 at 2148.730 kb on - strand, within Psyr_1873 at 2149.000 kb on - strand, within Psyr_1873 at 2149.004 kb on + strand, within Psyr_1873 at 2149.004 kb on + strand, within Psyr_1873 at 2149.005 kb on - strand, within Psyr_1873 at 2149.107 kb on + strand, within Psyr_1873 at 2149.107 kb on + strand, within Psyr_1873 at 2149.107 kb on + strand, within Psyr_1873 at 2149.107 kb on + strand, within Psyr_1873 at 2149.278 kb on + strand at 2149.316 kb on + strand at 2149.317 kb on - strand at 2149.351 kb on + strand at 2149.399 kb on + strand, within Psyr_1874 at 2149.399 kb on + strand, within Psyr_1874 at 2149.400 kb on - strand, within Psyr_1874 at 2149.400 kb on - strand, within Psyr_1874 at 2149.660 kb on + strand, within Psyr_1874 at 2149.674 kb on + strand, within Psyr_1874 at 2149.792 kb on + strand, within Psyr_1874 at 2149.792 kb on + strand, within Psyr_1874 at 2149.792 kb on + strand, within Psyr_1874 at 2149.793 kb on - strand, within Psyr_1874 at 2149.822 kb on + strand, within Psyr_1874 at 2149.852 kb on - strand, within Psyr_1874 at 2149.892 kb on - strand, within Psyr_1874 at 2149.925 kb on + strand, within Psyr_1874 at 2149.925 kb on + strand, within Psyr_1874 at 2149.925 kb on + strand, within Psyr_1874 at 2149.925 kb on + strand, within Psyr_1874 at 2149.926 kb on - strand, within Psyr_1874 at 2149.927 kb on + strand, within Psyr_1874 at 2149.928 kb on - strand, within Psyr_1874 at 2149.928 kb on - strand, within Psyr_1874 at 2149.928 kb on - strand, within Psyr_1874 at 2149.946 kb on + strand, within Psyr_1874 at 2149.993 kb on + strand, within Psyr_1874 at 2149.993 kb on + strand, within Psyr_1874 at 2149.993 kb on + strand, within Psyr_1874 at 2150.136 kb on + strand, within Psyr_1874 at 2150.137 kb on - strand, within Psyr_1874 at 2150.192 kb on + strand at 2150.193 kb on - strand at 2150.218 kb on + strand at 2150.218 kb on + strand at 2150.219 kb on - strand at 2150.219 kb on - strand at 2150.320 kb on - strand at 2150.465 kb on + strand, within Psyr_1875 at 2150.465 kb on + strand, within Psyr_1875 at 2150.465 kb on + strand, within Psyr_1875 at 2150.466 kb on - strand, within Psyr_1875 at 2151.040 kb on + strand at 2151.074 kb on - strand at 2151.113 kb on + strand at 2151.113 kb on + strand at 2151.213 kb on + strand at 2151.213 kb on + strand at 2151.214 kb on - strand at 2151.214 kb on - strand at 2151.214 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Avocatin A 10 mM remove 2,148,315 + +2.4 2,148,373 - -1.7 2,148,382 - +0.5 2,148,482 + -0.6 2,148,482 + -0.7 2,148,482 + -0.6 2,148,482 + -1.6 2,148,482 + -0.8 2,148,482 + -0.3 2,148,482 + +0.2 2,148,482 + +0.6 2,148,482 + -1.2 2,148,482 + +1.5 2,148,482 + -0.2 2,148,483 - -0.3 2,148,483 - +0.7 2,148,483 - +0.8 2,148,483 - +2.4 2,148,483 - +1.8 2,148,483 - -1.0 2,148,490 + +2.7 2,148,500 + -0.2 2,148,552 + +0.3 2,148,572 - Psyr_1873 0.12 +0.2 2,148,572 - Psyr_1873 0.12 -0.5 2,148,612 + Psyr_1873 0.18 -0.6 2,148,613 - Psyr_1873 0.18 -1.3 2,148,663 + Psyr_1873 0.25 -1.0 2,148,664 - Psyr_1873 0.25 -0.1 2,148,729 + Psyr_1873 0.34 +0.1 2,148,729 + Psyr_1873 0.34 -1.9 2,148,730 - Psyr_1873 0.34 +0.2 2,148,730 - Psyr_1873 0.34 -1.4 2,149,000 - Psyr_1873 0.71 -0.5 2,149,004 + Psyr_1873 0.71 +0.3 2,149,004 + Psyr_1873 0.71 -1.2 2,149,005 - Psyr_1873 0.72 +1.8 2,149,107 + Psyr_1873 0.86 -0.3 2,149,107 + Psyr_1873 0.86 -1.4 2,149,107 + Psyr_1873 0.86 +0.8 2,149,107 + Psyr_1873 0.86 +0.2 2,149,278 + -0.5 2,149,316 + +0.6 2,149,317 - +0.1 2,149,351 + -1.8 2,149,399 + Psyr_1874 0.13 +1.0 2,149,399 + Psyr_1874 0.13 -0.1 2,149,400 - Psyr_1874 0.13 +0.4 2,149,400 - Psyr_1874 0.13 -0.8 2,149,660 + Psyr_1874 0.40 +1.2 2,149,674 + Psyr_1874 0.41 +0.1 2,149,792 + Psyr_1874 0.53 -0.2 2,149,792 + Psyr_1874 0.53 +0.3 2,149,792 + Psyr_1874 0.53 -1.3 2,149,793 - Psyr_1874 0.53 -2.4 2,149,822 + Psyr_1874 0.56 +0.5 2,149,852 - Psyr_1874 0.59 -1.0 2,149,892 - Psyr_1874 0.63 -1.9 2,149,925 + Psyr_1874 0.67 -1.8 2,149,925 + Psyr_1874 0.67 +0.4 2,149,925 + Psyr_1874 0.67 -1.6 2,149,925 + Psyr_1874 0.67 +0.0 2,149,926 - Psyr_1874 0.67 -0.4 2,149,927 + Psyr_1874 0.67 -2.7 2,149,928 - Psyr_1874 0.67 -0.6 2,149,928 - Psyr_1874 0.67 +0.0 2,149,928 - Psyr_1874 0.67 -3.2 2,149,946 + Psyr_1874 0.69 +0.9 2,149,993 + Psyr_1874 0.74 +0.6 2,149,993 + Psyr_1874 0.74 -0.8 2,149,993 + Psyr_1874 0.74 -1.8 2,150,136 + Psyr_1874 0.89 -0.2 2,150,137 - Psyr_1874 0.89 -0.6 2,150,192 + -0.9 2,150,193 - -0.6 2,150,218 + -1.3 2,150,218 + -0.2 2,150,219 - +1.1 2,150,219 - -1.3 2,150,320 - +0.0 2,150,465 + Psyr_1875 0.14 +0.8 2,150,465 + Psyr_1875 0.14 +3.0 2,150,465 + Psyr_1875 0.14 +0.7 2,150,466 - Psyr_1875 0.14 -0.6 2,151,040 + -0.2 2,151,074 - -0.9 2,151,113 + +0.3 2,151,113 + -0.0 2,151,213 + -0.4 2,151,213 + -0.8 2,151,214 - +0.4 2,151,214 - -0.4 2,151,214 - -0.7
Or see this region's nucleotide sequence