Experiment: LB in EcoFAB_3.5mL with sand
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04220 and BWI76_RS04225 are separated by 57 nucleotides BWI76_RS04225 and BWI76_RS04230 are separated by 53 nucleotides
BWI76_RS04220: BWI76_RS04220 - two-component sensor histidine kinase, at 867,231 to 868,655
_RS04220
BWI76_RS04225: BWI76_RS04225 - cell envelope integrity protein CreD, at 868,713 to 870,071
_RS04225
BWI76_RS04230: BWI76_RS04230 - DNA-binding response regulator, at 870,125 to 870,841
_RS04230
Position (kb) 868
869
870
871 Strain fitness (log2 ratio) -2
-1
0
1 at 867.714 kb on + strand, within BWI76_RS04220 at 867.775 kb on + strand, within BWI76_RS04220 at 867.928 kb on + strand, within BWI76_RS04220 at 867.928 kb on + strand, within BWI76_RS04220 at 867.936 kb on - strand, within BWI76_RS04220 at 867.936 kb on - strand, within BWI76_RS04220 at 867.936 kb on - strand, within BWI76_RS04220 at 867.948 kb on + strand, within BWI76_RS04220 at 867.970 kb on + strand, within BWI76_RS04220 at 867.970 kb on + strand, within BWI76_RS04220 at 868.189 kb on + strand, within BWI76_RS04220 at 868.269 kb on + strand, within BWI76_RS04220 at 868.438 kb on + strand, within BWI76_RS04220 at 868.679 kb on + strand at 868.749 kb on + strand at 868.759 kb on + strand at 868.843 kb on - strand at 868.848 kb on - strand at 869.085 kb on - strand, within BWI76_RS04225 at 869.121 kb on + strand, within BWI76_RS04225 at 869.144 kb on + strand, within BWI76_RS04225 at 869.336 kb on + strand, within BWI76_RS04225 at 869.456 kb on - strand, within BWI76_RS04225 at 869.486 kb on + strand, within BWI76_RS04225 at 869.580 kb on + strand, within BWI76_RS04225 at 869.664 kb on - strand, within BWI76_RS04225 at 869.664 kb on - strand, within BWI76_RS04225 at 869.801 kb on - strand, within BWI76_RS04225 at 869.814 kb on - strand, within BWI76_RS04225 at 869.837 kb on + strand, within BWI76_RS04225 at 870.011 kb on + strand at 870.897 kb on - strand at 870.897 kb on - strand at 871.043 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB in EcoFAB_3.5mL with sand remove 867,714 + BWI76_RS04220 0.34 +0.2 867,775 + BWI76_RS04220 0.38 +0.4 867,928 + BWI76_RS04220 0.49 +1.0 867,928 + BWI76_RS04220 0.49 -1.0 867,936 - BWI76_RS04220 0.49 +0.2 867,936 - BWI76_RS04220 0.49 -1.5 867,936 - BWI76_RS04220 0.49 -0.9 867,948 + BWI76_RS04220 0.50 -0.4 867,970 + BWI76_RS04220 0.52 -0.6 867,970 + BWI76_RS04220 0.52 -1.8 868,189 + BWI76_RS04220 0.67 -0.1 868,269 + BWI76_RS04220 0.73 -0.5 868,438 + BWI76_RS04220 0.85 +0.7 868,679 + -0.6 868,749 + +0.0 868,759 + +0.2 868,843 - +0.6 868,848 - -0.1 869,085 - BWI76_RS04225 0.27 +0.9 869,121 + BWI76_RS04225 0.30 +1.3 869,144 + BWI76_RS04225 0.32 +0.5 869,336 + BWI76_RS04225 0.46 +0.1 869,456 - BWI76_RS04225 0.55 +1.1 869,486 + BWI76_RS04225 0.57 +0.5 869,580 + BWI76_RS04225 0.64 +0.2 869,664 - BWI76_RS04225 0.70 +0.2 869,664 - BWI76_RS04225 0.70 +0.6 869,801 - BWI76_RS04225 0.80 +0.3 869,814 - BWI76_RS04225 0.81 -0.1 869,837 + BWI76_RS04225 0.83 +1.0 870,011 + -0.6 870,897 - -0.2 870,897 - +0.8 871,043 - -0.2
Or see this region's nucleotide sequence