Experiment: Bu1_100
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt H281DRAFT_06404 and H281DRAFT_06405 overlap by 4 nucleotides H281DRAFT_06405 and H281DRAFT_06406 are separated by 46 nucleotides H281DRAFT_06406 and H281DRAFT_06407 overlap by 4 nucleotides H281DRAFT_06407 and H281DRAFT_06408 are separated by 375 nucleotides
H281DRAFT_06404: H281DRAFT_06404 - Predicted acyltransferases, at 1,461 to 2,015
_06404
H281DRAFT_06405: H281DRAFT_06405 - beta-lactamase class A, at 2,012 to 2,884
_06405
H281DRAFT_06406: H281DRAFT_06406 - D-alanyl-D-alanine endopeptidase (penicillin-binding protein 7), at 2,931 to 4,691
_06406
H281DRAFT_06407: H281DRAFT_06407 - Predicted transcriptional regulator, at 4,688 to 5,071
_06407
H281DRAFT_06408: H281DRAFT_06408 - transcriptional regulator, LacI family, at 5,447 to 6,466
_06408
Position (kb) 2
3
4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1.977 kb on + strand at 1.978 kb on - strand at 2.103 kb on + strand, within H281DRAFT_06405 at 2.103 kb on + strand, within H281DRAFT_06405 at 2.104 kb on - strand, within H281DRAFT_06405 at 2.154 kb on + strand, within H281DRAFT_06405 at 2.598 kb on + strand, within H281DRAFT_06405 at 2.598 kb on + strand, within H281DRAFT_06405 at 2.648 kb on + strand, within H281DRAFT_06405 at 3.371 kb on - strand, within H281DRAFT_06406 at 3.389 kb on - strand, within H281DRAFT_06406 at 3.728 kb on + strand, within H281DRAFT_06406 at 3.736 kb on + strand, within H281DRAFT_06406 at 3.737 kb on - strand, within H281DRAFT_06406 at 3.772 kb on + strand, within H281DRAFT_06406 at 3.772 kb on + strand, within H281DRAFT_06406 at 3.773 kb on - strand at 3.828 kb on + strand, within H281DRAFT_06406 at 3.829 kb on - strand, within H281DRAFT_06406 at 3.938 kb on + strand, within H281DRAFT_06406 at 3.939 kb on - strand, within H281DRAFT_06406 at 3.939 kb on - strand, within H281DRAFT_06406 at 4.064 kb on - strand, within H281DRAFT_06406 at 4.064 kb on - strand at 4.080 kb on - strand, within H281DRAFT_06406 at 4.213 kb on - strand, within H281DRAFT_06406 at 4.261 kb on + strand, within H281DRAFT_06406 at 4.261 kb on + strand, within H281DRAFT_06406 at 4.262 kb on - strand, within H281DRAFT_06406 at 4.262 kb on - strand, within H281DRAFT_06406 at 4.262 kb on - strand, within H281DRAFT_06406 at 4.262 kb on - strand, within H281DRAFT_06406 at 4.585 kb on - strand at 4.697 kb on - strand at 4.888 kb on + strand, within H281DRAFT_06407 at 4.888 kb on + strand, within H281DRAFT_06407 at 4.889 kb on - strand, within H281DRAFT_06407 at 4.889 kb on - strand, within H281DRAFT_06407 at 4.893 kb on - strand, within H281DRAFT_06407 at 4.893 kb on - strand, within H281DRAFT_06407 at 4.928 kb on + strand, within H281DRAFT_06407 at 4.928 kb on + strand, within H281DRAFT_06407 at 4.938 kb on + strand, within H281DRAFT_06407 at 4.938 kb on + strand, within H281DRAFT_06407 at 4.938 kb on + strand, within H281DRAFT_06407 at 4.938 kb on + strand, within H281DRAFT_06407 at 4.938 kb on + strand, within H281DRAFT_06407 at 4.942 kb on + strand, within H281DRAFT_06407 at 4.943 kb on - strand, within H281DRAFT_06407 at 4.943 kb on - strand, within H281DRAFT_06407 at 5.010 kb on - strand, within H281DRAFT_06407 at 5.108 kb on + strand at 5.370 kb on + strand at 5.370 kb on + strand at 5.445 kb on + strand at 5.445 kb on + strand at 5.445 kb on + strand at 5.448 kb on + strand at 5.466 kb on + strand at 5.467 kb on - strand at 5.577 kb on + strand, within H281DRAFT_06408 at 5.577 kb on + strand, within H281DRAFT_06408 at 5.635 kb on + strand, within H281DRAFT_06408 at 5.636 kb on - strand, within H281DRAFT_06408 at 5.637 kb on + strand, within H281DRAFT_06408 at 5.637 kb on + strand, within H281DRAFT_06408 at 5.637 kb on + strand, within H281DRAFT_06408 at 5.638 kb on - strand, within H281DRAFT_06408 at 5.638 kb on - strand, within H281DRAFT_06408
Per-strain Table
Position Strand Gene LocusTag Fraction Bu1_100 remove 1,977 + +0.7 1,978 - -0.2 2,103 + H281DRAFT_06405 0.10 +0.8 2,103 + H281DRAFT_06405 0.10 -1.2 2,104 - H281DRAFT_06405 0.11 +0.5 2,154 + H281DRAFT_06405 0.16 -0.9 2,598 + H281DRAFT_06405 0.67 +0.6 2,598 + H281DRAFT_06405 0.67 +1.7 2,648 + H281DRAFT_06405 0.73 +0.6 3,371 - H281DRAFT_06406 0.25 +0.4 3,389 - H281DRAFT_06406 0.26 +0.6 3,728 + H281DRAFT_06406 0.45 +0.3 3,736 + H281DRAFT_06406 0.46 +0.4 3,737 - H281DRAFT_06406 0.46 +0.0 3,772 + H281DRAFT_06406 0.48 +0.2 3,772 + H281DRAFT_06406 0.48 +1.5 3,773 - -0.3 3,828 + H281DRAFT_06406 0.51 +2.2 3,829 - H281DRAFT_06406 0.51 -1.2 3,938 + H281DRAFT_06406 0.57 -0.9 3,939 - H281DRAFT_06406 0.57 +0.5 3,939 - H281DRAFT_06406 0.57 +0.8 4,064 - H281DRAFT_06406 0.64 -1.0 4,064 - +1.7 4,080 - H281DRAFT_06406 0.65 -0.2 4,213 - H281DRAFT_06406 0.73 -1.5 4,261 + H281DRAFT_06406 0.76 -0.9 4,261 + H281DRAFT_06406 0.76 -0.1 4,262 - H281DRAFT_06406 0.76 -1.4 4,262 - H281DRAFT_06406 0.76 +0.5 4,262 - H281DRAFT_06406 0.76 +1.1 4,262 - H281DRAFT_06406 0.76 -0.4 4,585 - -1.6 4,697 - +0.8 4,888 + H281DRAFT_06407 0.52 -0.3 4,888 + H281DRAFT_06407 0.52 +1.1 4,889 - H281DRAFT_06407 0.52 -0.7 4,889 - H281DRAFT_06407 0.52 -2.2 4,893 - H281DRAFT_06407 0.53 +0.7 4,893 - H281DRAFT_06407 0.53 +0.6 4,928 + H281DRAFT_06407 0.62 +1.2 4,928 + H281DRAFT_06407 0.62 -3.1 4,938 + H281DRAFT_06407 0.65 +0.6 4,938 + H281DRAFT_06407 0.65 -0.3 4,938 + H281DRAFT_06407 0.65 +0.2 4,938 + H281DRAFT_06407 0.65 -0.2 4,938 + H281DRAFT_06407 0.65 -1.9 4,942 + H281DRAFT_06407 0.66 +0.3 4,943 - H281DRAFT_06407 0.66 +0.0 4,943 - H281DRAFT_06407 0.66 -0.5 5,010 - H281DRAFT_06407 0.84 -1.4 5,108 + -1.1 5,370 + -0.3 5,370 + -0.4 5,445 + -0.5 5,445 + +0.4 5,445 + +1.2 5,448 + -0.0 5,466 + +0.0 5,467 - +0.3 5,577 + H281DRAFT_06408 0.13 -2.2 5,577 + H281DRAFT_06408 0.13 -0.7 5,635 + H281DRAFT_06408 0.18 -2.0 5,636 - H281DRAFT_06408 0.19 +0.7 5,637 + H281DRAFT_06408 0.19 -0.5 5,637 + H281DRAFT_06408 0.19 +2.5 5,637 + H281DRAFT_06408 0.19 -0.3 5,638 - H281DRAFT_06408 0.19 -1.4 5,638 - H281DRAFT_06408 0.19 -0.3
Or see this region's nucleotide sequence